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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes
Journal of Clinical Microbiology, Volume 46, No. 2, Year 2008
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Description
A microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay (PCR-ELISA) has been used for the detection and identification of a variety of microorganisms. Here, we report the development of a PCR-ELISA for the identification of clinically relevant human rotavirus VP7 (G1 to G6, G8 to G10, and G12) and VP4 (P[4], P[6], P[8], P[9], and P[14]) genotypes. The G and P types of reference human and animal rotavirus strains for which specific probes were available were correctly identified by the PCR-ELISA. In addition, reference strains bearing G or P genotypes for which specific probes were unavailable, such as G11, G14, P[3], P[10], and P[11], did not display any cross-reactivity to the probes. The usefulness of the assay was further evaluated by analyzing a total of 396 rotavirus-positive stool samples collected in four countries: Brazil, Ghana, Japan, and the United States. The results of this study showed that the PCR-ELISA was sensitive and easy to perform without the use of any expensive and sophisticated equipment, the reagents used are easy to obtain commercially and advantageous over multiplex PCR since more than one type-specific probe is used and the selection of probes is more flexible. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Santos, Norma Suely De Oliveira
Brazil, Rio de Janeiro
Universidade Federal do Rio de Janeiro
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Honma, Shinjiro
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Timenetsky, Maria Do Carmo Sampaio Tavares Timenetsky
Brazil, Cerqueira Cesar
Instituto Adolfo Lutz
Linhares, Alexandre Da Costa
Brazil, Ananindeua
Instituto Evandro Chagas
Ushijima, Hiroshi
Japan, Tokyo
The University of Tokyo
Armah, George Enyimah
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Gentsch, Jon R.
United States, Atlanta
National Center for Immunization and Respiratory Diseases
Hoshino, Yatsutaka
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 32
Authors: 8
Affiliations: 7
Identifiers
Doi:
10.1128/JCM.01361-07
ISSN:
00951137
Research Areas
Genetics And Genomics
Study Locations
Ghana