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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids
Parasitology International, Volume 60, No. 1, Year 2011
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Description
We report the development of a colourimetric PCR/dot blot assay targeting the mitochondrial gene NADH dehydrogenase subunit 1 (nad1) for differential diagnosis of taeniid eggs. Partial sequences of the cestode nad1 gene were aligned and new primers were designed based on conserved regions. Species-specific oligonucleotide probes (S-SONP) for canine taeniid cestodes were then designed manually based on the variable region between the conserved primers. Specifically, S-SONP were designed for the Taenia crassiceps, T. hydatigena, T. multiceps, T. ovis, T. taeniaeformis, Echinococcus granulosus (genotype 1), E. multilocularis and E. vogeli. Each probe showed high specificity as no cross-hybridisation with any amplified nad1 fragment was observed. We evaluated the assay using 49 taeniid egg-positive samples collected from dogs in Zambia. DNA from 5 to 10 eggs was extracted in each sample. Using the PCR/dot blot assay, the probes successfully detected PCR products from T. hydatigena in 42 samples, T. multiceps in 3 samples, and both species (mixed infection) in the remaining 4 samples. The results indicate that the PCR/dot blot assay is a reliable alternative for differential diagnosis of taeniid eggs in faecal samples. © 2010 Elsevier Ireland Ltd.
Authors & Co-Authors
Armua-Fernandez, Maria Teresa
Japan, Sapporo
Graduate School of Veterinary Medicine
Nonaka, Nariaki
Japan, Miyazaki
University of Miyazaki
Sakurai, Tatsuya
Japan, Sapporo
Graduate School of Veterinary Medicine
Nakamura, Seita
Japan, Sapporo
Graduate School of Veterinary Medicine
Gottstein, Bruno B.
Switzerland, Bern
University of Bern
Deplazes, Peter
Switzerland, Zurich
Universität Zürich
Phiri, Isaac Khozozo
Zambia, Lusaka
University of Zambia
Katakura, Ken
Japan, Sapporo
Graduate School of Veterinary Medicine
Oku, Yuusaburo
Japan, Sapporo
Graduate School of Veterinary Medicine
Statistics
Citations: 21
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.parint.2010.11.005
ISSN:
13835769
Research Areas
Genetics And Genomics
Study Locations
Zambia