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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Proteomic analysis of the mature kernel aleurone layer in common and durum wheat
Journal of Cereal Science, Volume 55, No. 3, Year 2012
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Description
The aleurone layer (AL) is one of inner tissues removed from the grain with the wheat bran. It is the main source of vitamins, minerals and antioxidants of potential nutritional value in the wheat kernel. The AL of three varieties of each of the two main species of wheat, Triticum aestivum (ABD) and Triticum durum (AB), were manually dissected and analysed using two-dimensional gel-based proteomics. A total of 1258 and 1109 Coomassie-stained spots were detected in the AL of representatives of the ABD and AB genomes. In two varieties (T. aestivum Chinese Spring and T. durum Bidi17), grown in two different years with full fungicide protection, no quantitative or qualitative (presence/absence) differences in spots were detected, suggesting that AL proteome is strongly genetically controlled. Comparison within and between species revealed a total of 339 AL significant protein spots. Among these spots, 30.8% differed within T. aestivum and 56.5% within T. durum varieties, whereas only 12.7% differed between the two species. Among the 142 AL proteins identified using MALDI-TOF and LC-MS/MS, 57% were globulin type storage proteins (Glo-3, Glo-3B, Glo-3C, Glo-2), 16.2% were involved in carbohydrate metabolism and 17.6% in defence/stress pathways. These variations in AL proteome are discussed. © 2012 Elsevier Ltd.
Authors & Co-Authors
Meziani, Samira
France, Clermont-ferrand
Génétique Diversité et Ecophysiologie Des Céréales Gdec
Algeria, Sidi Bel Abbes
Université Djillali Liabes de Sidi Bel Abbes
Nadaud, I.
France, Clermont-ferrand
Génétique Diversité et Ecophysiologie Des Céréales Gdec
Gaillard-Martinie, Brigitte
France, Saint-genes-champanelle
Centre Clermont-auvergne-rhône-alpes
Chambon, Christophe
France, Saint-genes-champanelle
Centre Clermont-auvergne-rhône-alpes
Benali, Mohammed
Algeria, Sidi Bel Abbes
Université Djillali Liabes de Sidi Bel Abbes
Branlard, Gérard
France, Clermont-ferrand
Génétique Diversité et Ecophysiologie Des Céréales Gdec
Statistics
Citations: 34
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.jcs.2012.01.010
e-ISSN:
10959963
Study Approach
Qualitative
Quantitative