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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Intracellular localization and induction of a dynamic RNA-editing event of macro-algal V-ATPase subunit A (VHA-A) in response to copper
Plant, Cell and Environment, Volume 37, No. 1, Year 2014
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Description
A V-ATPase subunit A protein (VHA-A) transcript together with a variant (C793 to U), which introduces a stop codon truncating the subunit immediately downstream of its ATP binding site, was identified within a FucusvesiculosuscDNA from a heavy metal contaminated site. This is intriguing because the VHA-A subunit is the crucial catalytic subunit responsible for the hydrolysis of ATP that drives ion transport underlying heavy metal detoxification pathways. We employed a chemiluminescent hybridization protection assay to quantify the proportion of both variants directly from mRNA while performing quantification of total transcript using Q-PCR. Polyclonal antisera raised against recombinant VHA-A facilitated simultaneous detection of parent and truncated VHA-A and revealed its cellular and subcellular localization. By exploiting laboratory exposures and samples from an environmental copper gradient, we showed that total VHA-A transcript and protein, together with levels of the truncated variant, were induced by copper. The absence of a genomic sequence representing the truncated variant suggests a RNA editing event causing the production of the truncated VHA-A. Based on these observations, we propose RNA editing as a novel molecular process underpinning VHA trafficking and intracellular sequestration of heavy metals under stress. © 2013 John Wiley & Sons Ltd.
Authors & Co-Authors
Morris, Ceri A.
United Kingdom, Cardiff
College of Biomedical and Life Sciences
Owen, J. R.
United Kingdom, Cardiff
College of Biomedical and Life Sciences
Thomas, M. C.
United Kingdom, Cardiff
College of Biomedical and Life Sciences
El-Hiti, Gamal A.
Saudi Arabia, Riyadh
College of Applied Medical Sciences
Harwood, John L.
United Kingdom, Cardiff
College of Biomedical and Life Sciences
Kille, Peter
United Kingdom, Cardiff
College of Biomedical and Life Sciences
Statistics
Citations: 9
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1111/pce.12145
ISSN:
01407791
Research Areas
Genetics And Genomics