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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Cloning and expression analysis of a potato cDNA that encodes branching enzyme evidence for co-expression of starch biosynthetic genes
MGG Molecular & General Genetics, Volume 230, No. 1-2, Year 1991
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Description
One of the key enzymes involved in the formation of amylopectin, which is the major component of starch, is branching enzyme. A cDNA for potato branching enzyme was cloned by screening a tuber-specific cDNA expression library using an antiserum directed against a denatured preparation of the protein. Complementation of an Escherichia coli strain deficient in branching enzyme was achieved using a construct derived from this clone. Analysis of the expression of the gene in potato revealed a close association with conditions favouring starch biosynthesis. The expression pattern of the gene coding for potato branching enzyme, as analyzed at the mRNA level, closely resembles that of 4GPaseS, a gene coding for one of the subunits of ADP-glucose pyrophosphorylase, which is the key regulatory enzyme in the starch biosynthetic pathway. This raises the possibility that enzymes involved in the pathway are coordinately regulated at the transcriptional level. © 1991 Springer-Verlag.
Authors & Co-Authors
Kossmann, J. M.
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Visser, R. G.F.
Netherlands, Wageningen
Wageningen University & Research
Mueller-Roeber, Bernd
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Willmitzer, Lothar
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Sonnewald, Uwe
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Statistics
Citations: 110
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1007/BF00290648
ISSN:
16174623
Research Areas
Genetics And Genomics