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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of papanicolaou stain for studying micronuclei in buccal cells under field conditions
Acta Cytologica, Volume 50, No. 4, Year 2006
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Description
Objective: To compare Papanicolaou (Pap) and May-Grünwald Giemsa (MGG) stain as 2 techniques for staining for buccal mucosal cells to detect micronuclei (MN) infield studies. Study Design: Eighty cytologic smears (2 per individual) were taken from the buccal mucosa of 40 cigarette smokers recruited at a rural village in Egypt. Forty smears were stained with Pap stain and 40 with MGG stain. All were assessed for cellularity and scored for MN. Results: Pap stain was faster and easier to process and transport in the field study than was MGG stain. Regarding MGG smears, bacteria and cell debris masked the MN as compared to Pap smears, in which the fixative destroyed the bacteria and made the cell boundaries clearly demarcated. Using Pap stain, MN were seen easily in transparent cytoplasm. Conclusion: Pap stain is the preferred method infield studies for scoring and detecting MN in cells of buccal mucosa. © The International Academy of Cytology.
Authors & Co-Authors
Ayyad, Sohair B.A.
Egypt, Cairo
Ain Shams University Hospitals
Israel, Ebenezer
United States, Baltimore
University of Maryland, Baltimore Umb
El-Setouhy, M.
Egypt, Cairo
Faculty of Medicine - Ain Shams University
Radwan, Ghada Nasr
Egypt, Giza
Cairo University
Mohamed, Mostafa K.
Egypt, Cairo
Faculty of Medicine - Ain Shams University
Loffredo, Christopher A.
United States, Washington, D.c.
Georgetown University
Statistics
Citations: 31
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1159/000325980
ISSN:
00015547
Research Areas
Substance Abuse
Study Locations
Egypt