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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Amplification of rDNA loci to detect and type Neisseria meningitidis and other eubacteria
Molecular and Cellular Probes, Volume 7, No. 1, Year 1993
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Description
In 1991-92, Neisseria meningitidis group C was isolated from the blood of eight students in Urbana, Illinois, USA, and from the cerebrospinal fluid of one student from a nearby community, Decatur, Illinois. These and other bacterial species were analysed by PCR fingerprinting using primers selected from the ribosomal (r)DNA loci. A rDNA primer pair spanning a region within the 16S rDNA amplified a predicted 280 base pair (bp) DNA fragment from Neisseria spp. and fragments of different sizes for other genera. This primer pair specifically detected a carrier of N. meningitidis in a small clinical battery. Identity of the fragment was confirmed by restriction endonuclease analysis. A 600 bp fragment was also amplified from the 16S-23S internal transcribed spacer (ITS) of N. meningitidis; amplification from six other genera yielded different-sized fragments. Digestion of the ITS fragment from N. meningitidis with Alu I revealed three patterns; pattern I was found only for serogroup C isolates, and it was the dominant pattern among recent isolates with the exception of the one from Decatur. The isolate from Decatur yielded pattern III which suggested a non-clonal relationship to the seven isolates from Urbana. Patterns II and III were more prevalent in isolates from the 1960's and 1980's. PCR-based analysis of these loci can complement the techniques which are currently used for the detection and typing of these and other eubacteria. © 1993 Academic Press Limited.
Authors & Co-Authors
McLaughlin, Gerald Lee
United States, West Lafayette
Purdue University College of Veterinary Medicine
Howe, Daniel Keith
United States, West Lafayette
Purdue University College of Veterinary Medicine
Biggs, Diana R.
United States, Urbana-champaign
Carle Clinic Association
Smith, Arnold R.
United States, Urbana
University of Illinois Urbana-champaign
Ludwinski, Penny H.
United States, Champaign
Champaign - Urbana Public Health District
Fox, Barry C.
United States, Urbana
Carle Clinic
Tripathy, Deoki N.
United States, Urbana
University of Illinois Urbana-champaign
Frasch, Carl E.
United States, Silver Spring
Food and Drug Administration
Wenger, Jay D.
United States, Atlanta
National Center for Infectious Diseases
Carey, R. B.
United States, Evanston
St. Francis Hospital - Evanston
Hassan-King, Musa K.A.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Vodkin, Michael Harold
United States, West Lafayette
Purdue University College of Veterinary Medicine
United States, Urbana
University of Illinois Urbana-champaign
Statistics
Citations: 40
Authors: 12
Affiliations: 9
Identifiers
Doi:
10.1006/mcpr.1993.1002
ISSN:
08908508
Research Areas
Genetics And Genomics