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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The dosage dependence of VEGF stimulation on scaffold neovascularisation
Biomaterials, Volume 29, No. 26, Year 2008
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Description
Growth factors are often used in tissue regeneration to stimulate vascularisation of polymeric scaffolds, with vascular endothelial growth factor (VEGF) having been extensively studied for short-term vessel ingrowth. We have therefore evaluated the effect of different concentrations of VEGF on the vascularisation of a porous scaffold in the short-, intermediate- and long-term, by delivering 15, 150 and 1500 ng VEGF/day to polyurethane scaffolds by osmotic pumps for up to 6 weeks. An increased vascularisation months after termination of VEGF delivery was only achieved with 150 ng/day (46%, p < 0.05). This dosage consistently showed elevated levels of vascularisation (144, 125, 160 and 60% above PBS controls at 10, 20, 30 and 42 days, respectively, p < 0.05), whilst the vessels induced by the highest dosage, though initially maximally elevated (265 and 270% at 10 and 20 days, p < 0.05) tended to regress after 20 days of VEGF delivery. Pericyte coverage was decreased at 20 days for the highest dosage (30%, p < 0.05). Lectin perfusion demonstrated that vessels within the scaffold were connected to the host vasculature at all time points and perfusion was substantially raised by VEGF delivery at day 20. These results suggest concentration of VEGF plays a critical role in the nature and persistence of vasculature formed in a tissue regenerative scaffold. © 2008 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Davies, Neil Hamer
South Africa, Cape Town
University of Cape Town
Dobner, Stephan
South Africa, Cape Town
University of Cape Town
Bezuidenhout, Deon
South Africa, Cape Town
University of Cape Town
Schmidt, Christian Alexander P.
South Africa, Cape Town
University of Cape Town
Beck, Michael
United States, Minneapolis
Medtronic, Inc.
Zisch, Andreas Hugo
Switzerland, Zurich
Universitätsspital Zürich, Klinik Für Geburtshilfe
Switzerland, Zurich
Universität Zürich
Switzerland, Zurich
Eth Zürich
Zilla, Peter P.
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 95
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.biomaterials.2008.05.007
ISSN:
01429612
Research Areas
Health System And Policy