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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Investigation of viral etiology in potentially malignant disorders and oral squamous cell carcinomas in non-smoking, non-drinking patients
PLoS ONE, Volume 15, No. 4, Article e0232138, Year 2020
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Description
Head and neck squamous cell carcinomas (HNSCC) are the seventh most frequent cancers. Among HNSCCs, oral squamous cell carcinomas (OSCCs) include several anatomical locations of the oral cavity, but exclude the oropharynx. The known risk factors for OSCCs are mainly alcohol consumption and tobacco use for at least 75–80% of cases. In addition to these risk factors, Human papillomavirus (HPV) types 16 and 18, classified as high-risk (HR) HPV genotypes, are considered as risk factors for oropharyngeal cancers, but their role in the development of OSCC remains unclear. We tested the hypothesis of viral etiology in a series of 68 well-characterized OSCCs and 14 potentially malignant disorders (PMD) in non-smoking, non-drinking (NSND) patients using broad-range, sensitive molecular methodologies. Deep-sequencing of the transcriptome did not reveal any vertebrate virus sequences other than HPV transcripts, detected in only one case. In contrast, HPV DNA was detected in 41.2% (28/68) and 35.7% (5/14) of OSCC and PMD cases, respectively. Importantly, 90.9% (30/33) of these belonged to the Betapapillomavirus genus, but no viral transcripts were detected. Finally, high-throughput sequencing revealed reads corresponding to transcripts of the Trichomonas vaginalis virus (TVV), which were confirmed by RT-PCR in two OSCCs. Our results strongly suggest that Alphapapillomavirus genotypes classified as HR are not involved in the development of OSCCs in NSND patients and that known oncogenic infectious agents are absent in these specific OSCCs. Any possible direct or indirect role of Betapapillomavirus genus members and TVV in OSCCs remains speculative and requires further investigation. © 2020 Pérot et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Authors & Co-Authors
Pérot, Philippe
France, Paris
Institut Pasteur, Paris
Falguières, Michaël
France, Paris
Institut Pasteur, Paris
Arowas, Laurence
France, Paris
Institut Pasteur, Paris
Goudot, Patrick
France, Paris
Hôpital Universitaire Pitié Salpêtrière
Corre-Catelin, Nicole
France, Paris
Institut Pasteur, Paris
Ungeheuer, Marie Noëlle
France, Paris
Institut Pasteur, Paris
Caro, Valérie
France, Paris
Institut Pasteur, Paris
Heard, Isabelle
France, Paris
Institut Pasteur, Paris
Éloit, Marc E.
France, Paris
Institut Pasteur, Paris
France, Maisons-alfort
Ecole Nationale Vétérinaire D'alfort
Gessain, Antoine
France, Paris
Institut Pasteur, Paris
Bertolus, Chloé
France, Paris
Hôpital Universitaire Pitié Salpêtrière
France, Paris
Sorbonne Université
Berthet, Nicolas
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 11
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0232138
ISSN:
19326203
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Substance Abuse