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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Polysaccharides from the skin of the ray Raja radula. Partial characterization and anticoagulant activity
Thrombosis Research, Volume 123, No. 4, Year 2009
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Description
Introduction: The polysaccharide fraction from the skin of the ray Raja radula was extracted, characterized and assayed for anticoagulant activity. Materials and methods: A whole polysaccharidic fraction was extracted from the skin of the ray Raja radula by papain digestion followed by cetylpyridinium chloride and ethanol precipitation and was subjected to gel chromatography and anion exchange chromatography, acetate cellulose electrophoresis and characterized by physicochemical procedures. APTT and anti Xa assays were performed to assess the anticoagulant activity of the polysaccharidic fractions in comparison with unfractionated heparin. Results: Gel and anion-exchange chromatography revealed two negatively charged polysaccharidic populations different in both molecular weight and charge. Infrared spectra suggested the occurrence of uronic acids and acetylated hexosamines. The second polysaccharide was highly sulfated, with a sulfate content of approximately 29%. These data suggested that dermatan sulfate (DS) is the sulfate rich polysaccharide whereas hyaluronic acid (HA) is the polysaccharide devoid of sulfate groups. Molecular mass characterization indicated that their average molecular masses were 22 kDa and 85 kDa, respectively. The sulfated polysaccharide, i.e. presumably DS, accounted alone for the observed concentration-dependent anticoagulant activity which was, as measured by APTT, 2 to 3-fold lower than that of heparin. In addition, it had a significant anti-Xa activity. Conclusion: A major-sulfated polysaccharide, likely a dermatan sulfate, was extracted from the ray Raja radula skin. The results indicated that it exhibited a high anticoagulant activity and suggested that it was mediated by both heparin cofactor II and antithrombin. © 2008 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Ben Mansour, Mohamed
Tunisia, Monastir
Faculté de Médecine de Monastir
Majdoub, Hatem
Tunisia, Monastir
Faculté Des Sciences de Monastir
Bataille, Isabelle
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Roudesli, Mohamed Sadok
Tunisia, Monastir
Faculté Des Sciences de Monastir
Hassine, Mohssen
Tunisia, Monastir
Chu Fattouma-bourguiba
Ajzenberg, Nadine
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Chaubet, Frédéric
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Maaroufi, Raoui Mounir
Tunisia, Monastir
Faculté de Médecine de Monastir
Statistics
Citations: 51
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1016/j.thromres.2008.05.018
ISSN:
00493848