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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
High genetic diversity among Mycobacterium tuberculosis complex strains from Sierra Leone
BMC Microbiology, Volume 8, Article 103, Year 2008
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Description
Background. Among tuberculosis (TB) high incidence regions, Sub-Saharan Africa is particularly affected with approx. 1.6 million new cases every year. Besides this dramatic situation, data on the diversity of Mycobacterium tuberculosis complex (MTBC) strains causing this epidemic in this area are only sparsely available. Here we analyzed the population structure of strains from Sierra Leone with a special focus on the prevalence of M. africanum. Results. A total of 97 strains isolated from smear positive cases registered for re-treatment in the Western Area and Kenema districts in years 2003/2004 were investigated by susceptibility testing (first line drugs) and molecular typing (IS6110 fingerprinting, spoligotyping, and MIRU-VNTR typing). Among the strains analyzed, 32 were resistant to isoniazid, and 11 were multidrug resistant (at least resistant to isoniazid and rifampin). The population diversity was high with two previously described M. africanum lineages (West African-1, n = 6; West African-2, n = 17) and seven M. tuberculosis lineages (Haarlem, n = 14; LAM, n = 15; EAI, n = 4; Beijing, n = 4; S-type, n = 4, X-type, n = 1; Cameroon, n = 4). Furthermore, two new M. tuberculosis genotypes Sierra Leone-1 (n = 7) and -2 (n = 10) were found. Strain classification according to a 7 bp deletion in pks1/15 revealed that the majority of M. tuberculosis strains belonged to the Euro American lineage (66 out of 74). Conclusion. Resistance rates in Sierra Leone have reached an alarming level. The population structure of MTBC strains shows an intriguing diversity raising the question of possible consequences for TB epidemic and for the introduction of new diagnostic tests or treatment strategies in West Africa. © 2008 Homolka et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2447842/bin/1471-2180-8-103-S1.pdf
Authors & Co-Authors
Homolka, Susanne
Germany, Borstel
Forschungszentrum Borstel - Zentrum Für Medizin Und Biowissenschaften
Post, Erik
Germany, Wurzburg
German Leprosy and Tuberculosis Relief Association
Oberhauser, Barbara
Germany, Wurzburg
German Leprosy and Tuberculosis Relief Association
George, Abu Garawani
Sierra Leone
National Leprosy/tb Reference Laboratory
Westman, Lars
Sierra Leone
National Leprosy/tb Reference Laboratory
Dafae, Foday Mohamed
Sierra Leone
National Program Manager for Tuberculosis and Leprosy
Rüsch-Gerdes, Sabine
Germany, Borstel
Forschungszentrum Borstel - Zentrum Für Medizin Und Biowissenschaften
Niemann, Stefan
Germany, Borstel
Forschungszentrum Borstel - Zentrum Für Medizin Und Biowissenschaften
Statistics
Citations: 63
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1186/1471-2180-8-103
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Cohort Study
Study Locations
Multi-countries
Cameroon
Sierra Leone