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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
APOBEC-mediated interference with hepadnavirus production
Hepatology, Volume 42, No. 2, Year 2005
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Description
APOBEC3G is a cellular cytidine deaminase displaying broad antiretroviral activity. Recently, it was shown that APOBEC3G can also suppress hepatitis B virus (HBV) production in human hepatoma cells. In the present study, we characterized the mechanisms of APOBEC-mediated antiviral activity against HBV and related hepadnaviruses. We show that human APOBEC3G blocks HBV production in mammalian and nonmammalian cells and is active against duck HBV as well. Early steps of viral morphogenesis, including RNA and protein synthesis, binding of pregenomic RNA to core protein, and self-assembly of viral core protein, were unaffected. However, APOBEC3G rendered HBV core protein-associated full-length pregenomic RNA nuclease-sensitive. Ongoing reverse-transcription in capsids that had escaped the block in morphogenesis was not significantly inhibited. The antiviral effect was not modulated by abrogating or enhancing expression of the accessory HBVX protein, suggesting that HBVX protein does not represent a functional homologue to the HIV vif protein. Furthermore, human APOBEC3F but not rat APOBEC1 inhibited HBV DNA production. Viral RNA and low-level DNA produced in the presence of APOBEC3F or rat APOBEC1 occasionally displayed mutations, but the majority of clones were wild-type. In conclusion, APOBEC3G and APOBEC3F but not rat APOBEC1 can downregulate the production of replication-competent hepadnaviral nucleocapsids. In contrast to HIV and other retroviruses, however, APOBEC3G/3F-mediated editing of nucleic acids does not seem to represent an effective innate defense mechanism for hepadnaviruses. Copyright © 2005 by the American Association for the Study of Liver Diseases.
Authors & Co-Authors
Kock, Johan Lodewyk F.
Germany, Freiburg Im Breisgau
Universität Freiburg
Kann, Michael
Germany, Giessen
Justus-liebig-universität Gießen
Malim, Michael H.
United Kingdom, London
Faculty of Life Sciences & Medicine
Blum, Hubert Erich C.
Germany, Freiburg Im Breisgau
Universität Freiburg
Baumert, Thomas F.
Germany, Freiburg Im Breisgau
Universität Freiburg
von Weizsäcker, Fritz
Germany, Freiburg Im Breisgau
Universität Freiburg
Germany, Berlin
Schlosspark-klinik
Statistics
Citations: 128
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1002/hep.20801
ISSN:
02709139
Research Areas
Genetics And Genomics
Infectious Diseases