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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Aberrant DNase I digestion kinetics of nucleosomal core particles from sea urchin sperm
Biochemical and Biophysical Research Communications, Volume 155, No. 1, Year 1988
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Description
The pancreatic deoxyribonuclease (DNase I) digestion rates at the susceptible sites on nucleosomal core particles from blastula, gastrula and sperm cells of the sea urchin, Parechinus angulosus, have been determined. Although there are differences in their isohistone composition, the rates of digestion are similar for both embryonic stages. The rates of digestion for sperm core particles are 3-5 times lower than for embryo core particles at the more, and up to 2.5 times lower at the less susceptible sites. An explanation for these differences could be sought in the sperm isohistones H2B which are characterized by N-terminal extensions of 20-25 amino acid residues. © 1988 Academic Press, Inc.
Authors & Co-Authors
Landsman, David
South Africa, Cape Town
University of Cape Town
Trevor Sewell, B.
South Africa, Cape Town
University of Cape Town
von Holt, Claus
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 3
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/S0006-291X(88)81050-7
ISSN:
0006291X
e-ISSN:
10902104