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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to plasmodium falciparum
Journal of Leukocyte Biology, Volume 96, No. 6, Year 2014
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Description
Semi-immunity against Pf malaria is based on a combination of cellular and humoral immune responses. PMNs and IgGs are considered important components of this process, but the underlying mechanisms are unclear. We investigated the neutrophilic ADRB by analyzing the production of ROS in response to Pf antigenspecific IgGs bound to solid-phase immobilized antigens (sADRB) or whole merozoites (mADRB). We found that the PMN stimulations in each assay were based on different underlying mechanisms, demonstrating the importance of the assay set-up for the evaluation of antibody-triggered PMN responses. In the sADRB assay, ROS were produced externally, and by specific blocking of CD32(a)/FcγRII(a), the immediate neutrophilic response was abolished, whereas the removal of CD16(b)/FcγRIII(b) had no substantial effect. The key role of CD32(a) was confirmed using CD16(b)-deficient PMNs, in which similar changes of neutrophilic ADRB profiles were recorded after treatment. In the mADRB assay, ROS were produced almost exclusively within the cell, suggesting that the underlying mechanism was phagocytosis. This was confirmed using an additional phagocytosis assay, in which PMNs specifically ingested merozoites opsonized with Ghanaian plasma IgGs, seven times more often than merozoites opsonized with European plasma IgGs (P<0.001). Our data show that assay set-ups used to evaluate the responses of PMNs and perhaps other effector cells must be chosen carefully to evaluate the appropriate cellular responses. Our robust, stable, and well-characterized methods could therefore be useful in malaria vaccine studies to analyze the antimalarial effector function of antibodies. © Society for Leukocyte Biology.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4226792/bin/supp_96_6_1131__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC4226792/bin/supp_jlb.4A0614-283RR_jlb.4A0614-283RRSuppData.pdf
Authors & Co-Authors
Kapelski, Stephanie
Germany, Aachen
Fraunhofer Institute for Molecular Biology and Applied Ecology Ime
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Klockenbring, Torsten
Germany, Aachen
Fraunhofer Institute for Molecular Biology and Applied Ecology Ime
Fischer, Rainer
Germany, Aachen
Fraunhofer Institute for Molecular Biology and Applied Ecology Ime
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Barth, Stefan W.
Germany, Aachen
Fraunhofer Institute for Molecular Biology and Applied Ecology Ime
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Fendel, Rolf
Germany, Aachen
Fraunhofer Institute for Molecular Biology and Applied Ecology Ime
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Statistics
Citations: 33
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1189/jlb.4A0614-283RR
ISSN:
07415400
Research Areas
Infectious Diseases