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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Molecular characterization of glucose-6-phosphate dehydrogenase deficient variants in Baghdad city - Iraq
BMC Blood Disorders, Volume 12, Article 4, Year 2012
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Description
Background: Although G6PD deficiency is the most common genetically determined blood disorder among Iraqis, its molecular basis has only recently been studied among the Kurds in North Iraq, while studies focusing on Arabs in other parts of Iraq are still absent.Methods: A total of 1810 apparently healthy adult male blood donors were randomly recruited from the national blood transfusion center in Baghdad. They were classified into G6PD deficient and non-deficient individuals based on the results of methemoglobin reduction test (MHRT), with confirmation of deficiency by subsequent enzyme assays. DNA from deficient individuals was studied using a polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP) for four deficient molecular variants, namely G6PD Mediterranean (563 C→T), Chatham (1003 G→A), A- (202 G→A) and Aures (143 T→C). A subset of those with the Mediterranean variant, were further investigated for the 1311 (C→T) silent mutation.Results: G6PD deficiency was detected in 109 of the 1810 screened male individuals (6.0%). Among 101 G6PD deficient males molecularly studied, the Mediterranean mutation was detected in 75 cases (74.3%), G6PD Chatham in 5 cases (5.0%), G6PD A- in two cases (2.0%), and G6PD Aures in none. The 1311 silent mutation was detected in 48 out of the 51 G6PD deficient males with the Mediterranean variant studied (94.1%).Conclusions: Three polymorphic variants namely: the Mediterranean, Chatham and A-, constituted more than 80% of G6PD deficient variants among males in Baghdad. Iraq. This observation is to some extent comparable to other Asian Arab countries, neighboring Turkey and Iran. © 2012 Al-Musawi et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Al-Musawi, Bassam Musa Sadik
Iraq, Baghdad
University of Baghdad
Al-Allawi, Nasir A.S.
Iraq, Duhok
University of Duhok
Abdul-Majeed, Ban A.
Iraq, Baghdad
University of Baghdad
Eissa, Adil Abozaid
Iraq, Duhok
University of Duhok
Jubrael, Jaladet Mohammad Salih
Iraq, Duhok
University of Duhok
Hamamy, Hanan Ali
Switzerland, Geneva
Hôpitaux Universitaires de Genève
Statistics
Citations: 33
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1186/1471-2326-12-4
e-ISSN:
14712326
Research Areas
Cancer
Genetics And Genomics
Participants Gender
Male