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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A clone of the emergent Streptococcus pyogenes emm89 clade responsible for a large outbreak in a post-surgery oncology unit in France
Medical Microbiology and Immunology, Volume 207, No. 5-6, Year 2018
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Description
An outbreak of nosocomial infections due to Streptococcus pyogenes (Group A Streptococcus; GAS) occurred in a post-surgery oncology unit and concerned more than 60 patients and lasted 20 months despite enhanced infection control and prophylaxis measures. All GAS strains were characterized (emm genotype, toxin gene profile and pulse-field gel electrophoresis subtype). Selected strains were sequenced and phylogenetic relationship established. Capacity to form biofilm and interaction with human pulmonary epithelial cells and macrophages were determined. Twenty-six GAS strains responsible for invasive infections (II) and 57 for non-II or colonization were isolated from patients (n = 66) or healthcare workers (n = 13). Seventy strains shared the same molecular markers and 69 the same PFGE pattern; 56 were sequenced. They all belonged to the emerging emm89 clade 3; all but 1 were clonal. Whole genome sequencing identified 43 genetic profiles with sporadic mutations in regulatory genes and acquired mutations in 2 structural genes. Except for two regulatory gene mutants, all strains tested had the same biofilm formation capacity and displayed similar adherence and invasion of pulmonary epithelial cells and phagocytosis and survival in human macrophages. This large outbreak of GAS infection in a post-surgery oncology unit, a setting that contains highly susceptible patients, arose from a strain of the emergent emm89 clade. No relationship between punctual or acquired mutations, invasive status, and strain phenotypic characteristics was found. Noteworthy, the phenotypic characteristics of this clone account for its emergence and its remarkable capacity to elicit outbreaks. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Authors & Co-Authors
Plainvert, Céline
France, Paris
Inserm
France, Paris
Sorbonne Université
France, Paris
Institut Cochin
France, Paris
Hopital Cochin Ap-hp
Seringe, Élise
France, Paris
Ap-hp Assistance Publique - Hopitaux de Paris
Saintpierre, Benjamin
France, Paris
Inserm
France, Paris
Sorbonne Université
France, Paris
Institut Cochin
Sauvage, Elisabeth
France, Paris
Institut Pasteur, Paris
France, Paris
Cnrs Centre National de la Recherche Scientifique
Ma, Laurence
France, Paris
Institut Pasteur, Paris
Beghain, Johann
France, Paris
Institut Pasteur, Paris
Astagneau, Pascal P.
France, Paris
Ap-hp Assistance Publique - Hopitaux de Paris
Glaser, Philippe
France, Paris
Institut Pasteur, Paris
France, Paris
Cnrs Centre National de la Recherche Scientifique
Ariey, Frédéric
France, Paris
Inserm
France, Paris
Sorbonne Université
France, Paris
Institut Cochin
Poyart, Claire
France, Paris
Inserm
France, Paris
Sorbonne Université
France, Paris
Institut Cochin
France, Paris
Hopital Cochin Ap-hp
Statistics
Citations: 7
Authors: 10
Affiliations: 7
Identifiers
Doi:
10.1007/s00430-018-0546-1
ISSN:
03008584
Research Areas
Cancer
Genetics And Genomics
Health System And Policy