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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Germline recombination in a novel Cre transgenic line, Prl3b1-Cre mouse
Genesis, Volume 54, No. 7, Year 2016
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Description
Spermatogenesis is a complex and highly regulated process by which spermatogonial stem cells differentiate into spermatozoa. To better understand the molecular mechanisms of the process, the Cre/loxP system has been widely utilized for conditional gene knockout in mice. In this study, we generated a transgenic mouse line that expresses Cre recombinase under the control of the 2.5 kbp of the Prolactin family 3, subfamily b, member 1 (Prl3b1) gene promoter (Prl3b1-cre). Prl3b1 was initially reported to code for placental lactogen 2 (PL-2) protein in placenta along with increased expression toward the end of pregnancy. PL-2 was found to be expressed in germ cells in the testis, especially in spermatocytes. To analyze the specificity and efficiency of Cre recombinase activity in Prl3b1-cre mice, the mice were mated with reporter R26GRR mice, which express GFP ubiquitously before and tdsRed exclusively after Cre recombination. The systemic examination of Prl3b1-cre;R26GRR mice revealed that tdsRed-positive cells were detected only in the testis and epididymis. Fluorescence imaging of Prl3b1-cre;R26GRR testes suggested that Cre-mediated recombination took place in the germ cells with approximately 74% efficiency determined by in vitro fertilization. In conclusion, our results suggest that the Prl3b1-cre mice line provides a unique resource to understand testicular germ-cell development. genesis 54:389–397, 2016. © 2016 Wiley Periodicals, Inc.
Authors & Co-Authors
Al-Soudy, Al Sayed
Japan, Tsukuba
University of Tsukuba
Egypt, Giza
National Bank for Genes and Genetic Resources
Nakanishi, Tsuyoshi
Japan, Gifu
Gifu Pharmaceutical University
Mizuno, Seiya
Japan, Tsukuba
University of Tsukuba
Hasegawa, Yoshikazu
Japan, Tsukuba
University of Tsukuba
Shawki, Hossam H.
Japan, Tsukuba
University of Tsukuba
Egypt, Giza
National Bank for Genes and Genetic Resources
Katoh, Megumi C.
Japan, Tsukuba
University of Tsukuba
Basha, Walaa A.
Japan, Tsukuba
University of Tsukuba
Egypt, Ismailia
Faculty of Veterinary Medicine
Ibrahim, Abdelaziz E.
Japan, Tsukuba
University of Tsukuba
Egypt, Ismailia
Suez Canal University
El-Shemy, Hany A.
Egypt, Giza
Faculty of Agriculture
Iseki, Hiroyoshi
Japan, Tsukuba
University of Tsukuba
Yoshiki, Atsushi
Japan, Tsukuba
Riken Bioresource Research Center
Hiromori, Youhei
Japan, Gifu
Gifu Pharmaceutical University
Japan, Nagoya
Kinjo Gakuin University
Nagase, Hisamitsu
Japan, Gifu
Gifu Pharmaceutical University
Takahashi, Satoru
Japan, Tsukuba
University of Tsukuba
Oishi, Hisashi
Japan, Tsukuba
University of Tsukuba
Sugiyama, Fumihiro
Japan, Tsukuba
University of Tsukuba
Statistics
Citations: 16
Authors: 16
Affiliations: 8
Identifiers
Doi:
10.1002/dvg.22944
ISSN:
1526954X
e-ISSN:
1526968X
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health
Sexual And Reproductive Health