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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Rapid identification of malaria vaccine candidates based on α-helical coiled coil protein motif
PLoS ONE, Volume 2, No. 7, Article e645, Year 2007
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Description
To identify malaria antigens for vaccine development, we selected α-helical called coil domains of proteins predicted to be present in the parasite erythrocytic stage. The corresponding synthetic peptides are expected to mimic structurally "native" epitopes. Indeed the 95 chemically synthesized peptides were all specifically recognized by human immune sera though at various prevalence. Peptide specific antibodies were obtained both by affinity-purification from malaria immune sera and by immunization of mice. These antibodies did not show significant cross reactions, i.e., they were specific for the original prptide reacted with native parasite proteins in infected erythrocytes and several were active inhibiting in vivo parasite growth, circular dichroism studies indicated that the selected peptides assumed partial or high α-helical content. Thus, we demonstrate that the bioinformatics/ chemical synthesis approach described here can lead to the rapid identification of molecules which target biologically active antibodies, thus identifying suitable vaccine candidates. This strategy can be, in principle, extended to vaccine discovery in a wide range of other pathogens. © 2007 Villard et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1920550/bin/pone.0000645.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC1920550/bin/pone.0000645.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC1920550/bin/pone.0000645.s003.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC1920550/bin/pone.0000645.s004.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC1920550/bin/pone.0000645.s005.doc
Authors & Co-Authors
Villard, Viviane
Switzerland, Lausanne
Université de Lausanne Unil
Agak, George W.
Switzerland, Lausanne
Université de Lausanne Unil
Frank, Geraldine
Switzerland, Lausanne
Université de Lausanne Unil
Jafarshad, Ali
France, Paris
Institut Pasteur, Paris
Servis, Catherine
Switzerland, Lausanne
Université de Lausanne Unil
Nèbiè, Issa O.
Burkina Faso, Ouagadougou
Centre National de Recherche et de Formation Sur le Paludisme
Sirima, Sodiomon Bienvenu
Burkina Faso, Ouagadougou
Centre National de Recherche et de Formation Sur le Paludisme
Felger, Ingrid
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Arev́alo-Herrera, Myriam
Colombia, Cali
Universidad Del Valle, Cali
Herrera, Sócrates V.
Colombia, Cali
Universidad Del Valle, Cali
Heitz, Frédéric
France, Montpellier
Centre de Recherche en Biologie Cellulaire de Montpellier
Bäcker, Volker
France, Montpellier
Centre de Recherche en Biologie Cellulaire de Montpellier
Druilhe, Pierre L.
France, Paris
Institut Pasteur, Paris
Kajava, Andrey V.
France, Montpellier
Centre de Recherche en Biologie Cellulaire de Montpellier
Corradin, Giampietro
Switzerland, Lausanne
Université de Lausanne Unil
Statistics
Citations: 81
Authors: 15
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0000645
e-ISSN:
19326203
Research Areas
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study