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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Aluminium reduces sugar uptake in tobacco cell cultures: A potential cause of inhibited elongation but not of toxicity
Journal of Experimental Botany, Volume 61, No. 6, Year 2010
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Description
Aluminium is well known to inhibit plant elongation, but the role in this inhibition played by water relations remains unclear. To investigate this, tobacco (Nicotiana tabacum L.) suspension-cultured cells (line SL) was used, treating them with aluminium (50 μM) in a medium containing calcium, sucrose, and MES (pH 5.0). Over an 18 h treatment period, aluminium inhibited the increase in fresh weight almost completely and decreased cellular osmolality and internal soluble sugar content substantially; however, aluminium did not affect the concentrations of major inorganic ions. In aluminium-treated cultures, fresh weight, soluble sugar content, and osmolality decreased over the first 6 h and remained constant thereafter, contrasting with their continued increases in the untreated cultures. The rate of sucrose uptake, measured by radio-tracer, was reduced by approximately 60% within 3 h of treatment. Aluminium also inhibited glucose uptake. In an aluminium-tolerant cell line (ALT301) isogenic to SL, all of the above-mentioned changes in water relations occurred and tolerance emerged only after 6 h and appeared to involve the suppression of reactive oxygen species. Further separating the effects of aluminium on elongation and cell survival, sucrose starvation for 18 h inhibited elongation and caused similar changes in cellular osmolality but stimulated the production of neither reactive oxygen species nor callose and did not cause cell death. We propose that the inhibition of sucrose uptake is a mechanism whereby aluminium inhibits elongation, but does not account for the induction of cell death. © 2010 The Author(s).
Authors & Co-Authors
Abdel-Basset, Refaat M.
Japan, Okayama
Okayama University
Egypt, Asyut
Faculty of Science
Ozuka, Shotaro
Japan, Okayama
Okayama University
Demiral, Tijen
Japan, Okayama
Okayama University
Turkey, Izmir
Ege Üniversitesi
Turkey, Sanliurfa
Harran Üniversitesi
Furuichi, Takuya
Japan, Nagoya
Nagoya University Graduate School of Medicine
Japan, Okayama
Okayama University
Sawatani, Ikuo
Japan, Okayama
Hayashibara Group
Baskin, Tobias I.
United States, Amherst
University of Massachusetts Amherst
Matsumoto, Hideaki
Japan, Okayama
Okayama University
Yamamoto, Yoko
Japan, Okayama
Okayama University
Statistics
Citations: 25
Authors: 8
Affiliations: 7
Identifiers
Doi:
10.1093/jxb/erq027
ISSN:
00220957
e-ISSN:
14602431
Research Areas
Environmental
Substance Abuse