Simple method for transformation of Ochrobactrum anthropi

A rapid and simple method for preparation of highly efficient Ochrobactrum anthropi electrocompetent cells has been developed. The efficiency of transformation increased 200-fold when the cells were prepared from liquid culture compared to agar plates. Effects of different conditions, including cell density, electric field strength, resistance and plasmid size were evaluated to develop an electroporation protocol. The electrocompetent O. anthropi prepared by this method were 9-fold more efficient than commercial sources of competent Escherichia coli. The method described here will enhance the genetic manipulation of Ochrobactrum as a bioremediation tool and a biopesticide agent. © 2008 Springer Science+Business Media B.V.