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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Multilaboratory approach to preclinical evaluation of vaccine immunogens for placental malaria
Infection and Immunity, Volume 81, No. 2, Year 2013
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Description
Pregnancy malaria is caused by Plasmodium falciparum-infected erythrocytes that adhere to the placental receptor chondroitin sulfate A (CSA) and sequester in the placenta; women become resistant to pregnancy malaria as they acquire antiadhesion antibodies that target surface proteins of placental parasites. VAR2CSA, a member of the P. falciparum EMP1 variant surface antigen family, is the leading candidate for a pregnancy malaria vaccine. Because VAR2CSA is a high-molecular-weight protein, a vaccine based on the full-length protein may not be feasible. An alternative approach has been to develop a vaccine targeting individual Duffy binding-like (DBL) domains. In this study, a consortium of laboratories under the Pregnancy Malaria Initiative compared the functional activity of antiadhesion antibodies elicited by different VAR2CSA domains and variants produced in prokaryotic and eukaryotic expression systems. Antisera were initially tested against laboratory lines of maternal parasites, and the most promising reagents were evaluated in the field against fresh placental parasite samples. Recombinant proteins expressed in Escherichia coli elicited antibody levels similar to those expressed in eukaryotic systems, as did the two allelic forms of the DBL4 and DBL5 domains. The procedures developed for this head-to-head comparison will be useful for future evaluation and down-selection of malaria vaccine immunogens. ©2013, American Society for Microbiology.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3553800/bin/supp_81_2_487__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3553800/bin/IAI.01106-12_zii999099986so1.pdf
Authors & Co-Authors
Fried, Michal W.
United States, Seattle
Seattle Biomedical Research Institute
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Avril, Marion
United States, Seattle
Seattle Biomedical Research Institute
Chaturvedi, Richa
United States, Seattle
Seattle Biomedical Research Institute
Fernandez, Pablo
France, Paris
Institut Pasteur, Paris
France, Paris
Cnrs Centre National de la Recherche Scientifique
Lograsso, Joseph
United States, Seattle
Seattle Biomedical Research Institute
Narum, David L.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Nielsen, M. A.
Denmark, Copenhagen
Københavns Universitet
Oleǐnikov, Andrew V.
United States, Seattle
Seattle Biomedical Research Institute
Resende, Mafalda
Denmark, Copenhagen
Københavns Universitet
Salanti, Ali
Denmark, Copenhagen
Københavns Universitet
Saveria, Tracy
United States, Seattle
Seattle Biomedical Research Institute
Williamson, Kathryn
United States, Seattle
Seattle Biomedical Research Institute
Dicko, Alassane A.
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Scherf, Artur
France, Paris
Institut Pasteur, Paris
France, Paris
Cnrs Centre National de la Recherche Scientifique
Smith, Joseph D.
United States, Seattle
Seattle Biomedical Research Institute
G. Theander, Thor Grundtvig
Denmark, Copenhagen
Københavns Universitet
Duffy, Patrick Emmet
United States, Seattle
Seattle Biomedical Research Institute
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 36
Authors: 17
Affiliations: 6
Identifiers
Doi:
10.1128/IAI.01106-12
ISSN:
00199567
e-ISSN:
10985522
Research Areas
Infectious Diseases
Maternal And Child Health
Sexual And Reproductive Health
Participants Gender
Female