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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Galectin-1 induced activation of the apoptotic death-receptor pathway in human Jurkat T lymphocytes
Histochemistry and Cell Biology, Volume 129, No. 5, Year 2008
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Description
Galectin-1 (gal-1), a member of the family of β-galactoside binding proteins, participates in several biological processes such as immunomodulation, cell adhesion, regulation of cell growth and apoptosis. The aim of this study was to investigate whether gal-1 interferes with the Fas (Apo-1/CD95)-associated apoptosis cascade in the T-cell lines Jurkat and MOLT-4. Gal-1 and an Apo-1 monoclonal antibody (mAb) induced DNA-fragmentation in Jurkat T-cells whereas MOLT-4 cells were resistant. Gal-1 stimulated DNA-fragmentation could be efficiently inhibited by caspase-8 inhibitor II (Z-IETD-FMK) and a neutralizing Fas mAb. Fas could be identified as a target for gal-1 recognition as demonstrated by immunofluorescence staining, binding of the receptor glycoprotein to immobilized gal-1 and analyses by immunoblotting as well as by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Gal-1 stimulates the activation and proteolytic processing of procaspase-8 and downstream procaspase-3 in Jurkat-T cells. Inhibition of gal-1 induced procaspase-8 activation by a neutralizing Fas mAb strongly suggests that gal-1 recognition of Fas is associated with caspase-8 activation. Our data provide the first experimental evidence for targeting of gal-1 to glycotopes on Fas and the subsequent activation of the apoptotic death-receptor pathway. © 2008 Springer-Verlag.
Authors & Co-Authors
Brandt, Bettina
Germany, Rostock
Universität Rostock
Büchse, Tom
Germany, Rostock
Universität Rostock
Abou-Eladab, Ehab F.G.
Germany, Rostock
Universität Rostock
Egypt, Mansoura
Mansoura University
Tiedge, Markus
Germany, Rostock
Universität Rostock
Krause, Eberhard G.
Germany, Berlin
Leibniz-institut Für Molekulare Pharmakologie
Jeschke, Udo
Germany, Munich
Ludwig-maximilians-universität München
Walzel, Hermann
Germany, Rostock
Universität Rostock
Statistics
Citations: 50
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1007/s00418-008-0395-x
ISSN:
09486143
Research Areas
Cancer
Genetics And Genomics