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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species
Journal of Clinical Microbiology, Volume 47, No. 1, Year 2009
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Description
Rapid detection and differentiation of Taenia species are required for the control and prevention of taeniasis and cysticercosis in areas where these diseases are endemic. Because of the lower sensitivity and specificity of the conventional diagnosis based on microscopical examination, molecular tools are more reliable for differential diagnosis of these diseases. In this study, we developed and evaluated a loop-mediated isothermal amplification (LAMP) assay for differential diagnosis of infections with Taenia species with cathepsin L-like cysteine peptidase (clp) and cytochrome c oxidase subunit 1 (cox1) genes. LAMP with primer sets to the cox1 gene could differentiate between three species, and LAMP with primer sets to the clp gene could differentiate Taenia solium from Taenia saginata/Taenia asiatica. Restriction enzyme digestion of the LAMP products from primer set Tsag-clp allowed the differentiation of Taenia saginata from Taenia asiatica. We demonstrated the high specificity of LAMP by testing known parasite DNA samples extracted from proglottids (n = 100) and cysticerci (n = 68). LAMP could detect one copy of the target gene or five eggs of T. asiatica and T. saginata per gram of feces, showing sensitivity similar to that of PCR methods. Furthermore, LAMP could detect parasite DNA in all taeniid egg-positive fecal samples (n = 6). Due to the rapid, simple, specific, and sensitive detection of Taenia species, the LAMP assays are valuable tools which might be easily applicable for the control and prevention of taeniasis and cysticercosis in countries where these diseases are endemic. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Nkouawa, Agathe
Japan, Asahikawa
Asahikawa Medical University
Cameroon, Yaounde
Institute of Medical Research and Medicinal Plant Studies Yaounde
Sako, Yasuhito
Japan, Asahikawa
Asahikawa Medical University
Nakao, Minoru
Japan, Asahikawa
Asahikawa Medical University
Nakaya, Kazuhiro
Japan, Asahikawa
Asahikawa Medical University
Ito, Akira
Japan, Asahikawa
Asahikawa Medical University
Statistics
Citations: 116
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1128/JCM.01573-08
ISSN:
00951137
Research Areas
Genetics And Genomics