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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
A comparative analysis of gene expression patterns and cell phenotypes between cervical and peripheral blood mononuclear cells
PLoS ONE, Volume 4, No. 12, Article e8293, Year 2009
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Description
Studies of the immunological environment in the female genital tract (FGT) are critical for the development of vaccines or microbicides to halt the spread of sexually transmitted infections. Challenges arise due to the difficulties of sampling from this site, and the majority of studies have been conducted utilising peripheral blood mononuclear cells. Identifying functional differences between immune cells of the FGT and peripheral blood would aid in our understanding of mucosal immunology. We compared the gene expression profile of mononuclear cells at these two sites. Messenger RNA expression analysis was performed using gene expression arrays on matched cervical mononuclear cells and peripheral blood mononuclear cells. Further cellular phenotyping was done by 10 colour flow cytometry. Of the 22,185 genes expressed by these samples, 5345 genes were significantly differentially expressed between the cell populations. Most differences can be explained by significantly lower levels of T and B cells and higher levels of macrophages and dendritic cells in the FGT compared with peripheral blood. Several immunologically relevant pathways such as apoptosis and innate immune signalling, and a variety of cytokines and cytokine receptors were differentially expressed. This study highlights the importance of the unique immunological environment of the FGT and identifies important differences between systemic and mucosal immune compartments. © 2009 Horton et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2790076/bin/pone.0008293.s001.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC2790076/bin/pone.0008293.s002.doc
Authors & Co-Authors
Horton, Rachel E.
Canada, Winnipeg
University of Manitoba
Australia, Brisbane
Griffith University
Kaefer, Nadine
Canada, Winnipeg
University of Manitoba
Songok, Elijah Maritim
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
Kenya Medical Research Institute
Guijón, Fernando B.
Canada, Winnipeg
University of Manitoba
Kettaf, Nadia
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Boucher, Genèvieve
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Seḱaly, Rafick Pierre
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Blake Ball, T.
Canada, Winnipeg
University of Manitoba
Canada, Ottawa
Public Health Agency of Canada
Plummer, Francis Allan
Canada, Winnipeg
University of Manitoba
Canada, Winnipeg
National Microbiology Laboratory
Statistics
Citations: 9
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0008293
e-ISSN:
19326203
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health
Participants Gender
Female