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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Characterization of Escherichia coli from raw poultry in Belgium and impact on the detection of Campylobacter jejuni using Bolton broth
International Journal of Food Microbiology, Volume 135, No. 3, Year 2009
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Description
A comparative study examining Bolton broth and Preston broth for enrichment and reliable detection of Campylobacter jejuni (both healthy and freeze stressed cells) was performed. Tested as pure cultures, Bolton broth enabled faster resuscitation and growth of C. jejuni compared to Preston broth. When C. jejuni was co-incubated with extended-spectrum-beta-lactamase (ESBL) producing Escherichia coli isolated from Belgian poultry meat preparations, the latter dominated in the Bolton enrichment broth and crowded the mCCDA plates. This resulted in the inability to recover C. jejuni by ISO 10272-1:2006 standard method. Preston broth did not support the growth of the ESBL E. coli isolates, but showed longer detection time of C. jejuni compared to Bolton broth. The use of the same antibiotic (sodium cefoperazone) in Bolton broth and in mCCDA plates may explain the problems encountered for detection of C. jejuni, as high numbers of ESBL E. coli present after enrichment in Bolton broth, also caused overgrowth and masked the few C. jejuni colonies present on the mCCDA plates. The use of Campylobacter spp. specific real-time PCR circumvented these problems and enabled rapid detection of the pathogen after 24 h enrichment in both Bolton and Preston broth, for both healthy and freeze stressed cells. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Jasson, Vicky
Belgium, Ghent
Laboratory of Food Microbiology and Food Preservation
Sampers, Imca A.
Belgium, Ghent
Laboratory of Food Microbiology and Food Preservation
Belgium, Kortrijk
Hogeschool West-vlaanderen
Botteldoorn, Nadine
Belgium
Institute of Public Health - Iph
López-Gálvez, Francisco
Spain, Murcia
Research Group on Quality
Baert, Leen
Belgium, Ghent
Laboratory of Food Microbiology and Food Preservation
Denayer, Sarah
Belgium
Institute of Public Health - Iph
Rajković, Andreja
Belgium, Ghent
Laboratory of Food Microbiology and Food Preservation
Habib, Ihab
Belgium, Ghent
Universiteit Gent
Egypt, Alexandria
High Institute of Public Health
de Zutter, Lieven
Belgium, Ghent
Universiteit Gent
Debevere, Johan M.
Belgium, Ghent
Laboratory of Food Microbiology and Food Preservation
Uyttendaele, Mieke R.
Belgium, Ghent
Laboratory of Food Microbiology and Food Preservation
Statistics
Citations: 65
Authors: 11
Affiliations: 6
Identifiers
Doi:
10.1016/j.ijfoodmicro.2009.09.007
ISSN:
01681605