Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Hydroxytyrosol induces proliferation and cytoprotection against oxidative injury in vascular endothelial cells: Role of Nrf2 activation and HO-1 induction
Journal of Agricultural and Food Chemistry, Volume 59, No. 9, Year 2011
Notification
URL copied to clipboard!
Description
Hydroxytyrosol (HT), a phenolic compound in olive oil and leaves, has been reported to prevent various human pathologies including cardiovascular diseases. This study investigated the effects of HT on proliferation and protection against oxidative stress-induced damage in vascular endothelial cells (VECs) and the molecular mechanism(s) involved. Treatment of VECs withHTincreased cell proliferation, promoted wound repair, and protected cells againstH 2O2 cytotoxicity through the activation of Akt and ERK1/2, but not p38 MAPK. HT increased the expression and nuclear translocation of nuclear factor-E2-related factor-2 (Nrf2). Nrf2 expression was attenuated by LY294002 and U0126, inhibitors of phosphatidylinositol-3-kinase and MEK1/2, respectively. Nrf2 siRNA decreased both proliferative and cytoprotective effects of HT and abrogated HO-1 induction. Moreover, HO-1 inhibition with HO-1 siRNA or zinc protoporphyrin IX significantly prevented HT-induced cell proliferation, cytoprotection, and reduction in intracellular reactive oxygen species (ROS), suggesting that HO-1 is involved in these HT functions. The findings demonstrate that HT positively regulates the antioxidant defense system in VECs through the activation of Nrf2 followed by cell proliferation and resistance to vascular injury. The present study provides a molecular basis for the contribution of HT in the Mediterranean diet to the prevention of cardiovascular diseases. © 2011 American Chemical Society.
Authors & Co-Authors
Zrelli, Houda
Japan, Tsukuba
University of Tsukuba
Matsuoka, Mieko
Japan, Tsukuba
University of Tsukuba
Kitazaki, Shiho
Japan, Tsukuba
University of Tsukuba
Araki, Masahiro
Japan, Tsukuba
University of Tsukuba
Kusunoki, Miki
Japan, Tsukuba
University of Tsukuba
Zarrouk, Mokhtar
Tunisia, Hamam Lif
High Institute of Environmental Science and Technology Hiest of Borj Cedria
Miyazaki, Hitoshi
Japan, Tsukuba
University of Tsukuba
Statistics
Citations: 100
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1021/jf104151d
ISSN:
00218561
e-ISSN:
15205118
Research Areas
Cancer
Noncommunicable Diseases
Violence And Injury