Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Rapid platelet turnover in WASP(-) mice correlates with increased ex vivo phagocytosis of opsonized WASP(-) platelets
Experimental Hematology, Volume 36, No. 5, Year 2008
Notification
URL copied to clipboard!
Description
Objective: Our objective was to determine a mechanism for the thrombocytopenia of murine Wiskott-Aldrich syndrome (WAS). Materials and Methods: Consumption rates of WAS protein (WASP)(-) and wild-type (WT) platelets were measured by injection of 5-chloromethylfluorescein diacetate (CMFDA)-labeled platelets into WT or WASP(-) recipients, and by in vivo biotinylation. Platelet and reticulated platelet counts were performed using quantitative flow cytometry. Bone marrow megakaryocyte number and ploidy was assessed by flow cytometry. Phagocytosis of CMFDA-labeled, opsonized platelets was assessed using bone marrow-derived macrophages. Serum antiplatelet antibodies were assayed via their binding to WT platelets. Results: CMFDA-labeled WASP(-) platelets are consumed more rapidly than WT platelets in either WT or WASP(-) recipients. In vivo biotinylation studies corroborate these findings and show a normal consumption rate for WASP(-) reticulated platelets. The number of reticulated platelets is reduced in WASP(-) mice, but a significant number of the mice show an increased proportion of reticulated platelets and more severe thrombocytopenia. Sera from some of the latter group contain antiplatelet antibodies. Compared to WT platelets, WASP(-) platelets opsonized with anti-CD61 or 6A6 antibody are taken up more rapidly by bone marrow-derived macrophages. In vivo consumption rates of WASP(-) platelets are more accelerated by opsonization than are those of WT platelets. Conclusion: Both rapid clearance and impaired production contribute to the thrombocytopenia of murine WAS. Increased susceptibility of opsonized WASP(-) platelets to phagocytosis leads to increased in vivo clearance. This correlates with a higher incidence of individuals with an elevated fraction of reticulated platelets, a more severe thrombocytopenia, and antiplatelet antibodies. © 2008 ISEH - Society for Hematology and Stem Cells.
Authors & Co-Authors
Prislovsky, Amanda
United States, Memphis
Memphis va Medical Center
Marathe, Bindumadhav M.
United States, Memphis
University of Tennessee Health Science Center
Hosni, Amira
United States, Memphis
Memphis va Medical Center
Egypt, Fayoum
Faculty of Science
Bolen, Alyssa L.
United States, Memphis
University of Tennessee Health Science Center
Nimmerjahn, F.
Germany, Erlangen
Medizinische Fakultät
Jackson, Carl W.
United States, Memphis
St. Jude Children's Research Hospital
Weiman, Darryl S.
United States, Memphis
Memphis va Medical Center
Strom, Ted S.
United States, Memphis
Memphis va Medical Center
United States, Memphis
University of Tennessee Health Science Center
Statistics
Citations: 50
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1016/j.exphem.2007.12.019
ISSN:
0301472X
Research Areas
Cancer
Study Design
Cohort Study
Study Approach
Quantitative