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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
An international collaboration to standardize HIV-2 viral load assays: Results from the 2009 ACHI
E
V
2E
quality control study
Journal of Clinical Microbiology, Volume 49, No. 10, Year 2011
Notification
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Description
Accurate HIV-2 plasma viral load quantification is crucial for adequate HIV-2 patient management and for the proper conduct of clinical trials and international cohort collaborations. This study compared the homogeneity of HIV-2 RNA quantification when using HIV-2 assays from ACHI EV 2E study sites and either in-house PCR calibration standards or common viral load standards supplied to all collaborators. Each of the 12 participating laboratories quantified blinded HIV-2 samples, using its own HIV-2 viral load assay and standard as well as centrally validated and distributed common HIV-2 group A and B standards (http://www.hiv .lanl.gov/content/sequence/ HelpDocs/subtypes-more.html). Aliquots of HIV-2 group A and B strains, each at 2 theoretical concentrations (2.7 and 3.7 log 10 copies/ml), were tested. Intralaboratory, interlaboratory, and overall variances of quantification results obtained with both standards were compared using F tests. For HIV-2 group A quantifications, overall and interlaboratory and/or intralaboratory variances were significantly lower when using the common standard than when using in-house standards at the concentration levels of 2.7 log 10 copies/ml and 3.7 log 10 copies/ml, respectively. For HIV-2 group B, a high heterogeneity was observed and the variances did not differ according to the type of standard used. In this international collaboration, the use of a common standard improved the homogeneity of HIV-2 group A RNA quantification only. The diversity of HIV-2 group B, particularly in PCR primer-binding regions, may explain the heterogeneity in quantification of this strain. Development of a validated HIV-2 viral load assay that accurately quantifies distinct circulating strains is needed. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Damond, Florence
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Bénard, Antoine
France, Paris
Inserm
Balotta, Claudia
Italy, Milan
Ospedale Luigi Sacco - Polo Universitario
Böni, Jürg
Switzerland, Zurich
Universität Zürich
Cotten, Matt
Gambia, Banjul
Medical Research Council Laboratories Gambia
Duque, Vítor
Portugal, Coimbra
Centro Hospitalar e Universitário de Coimbra
Ferns, Bridget
United Kingdom, London
Ucl Medical School
Garson, Jeremy A.
United Kingdom, London
Ucl Medical School
Gomes, Perpétua C.
Portugal, Lisbon
Centro Hospitalar de Lisboa Ocidental Hospital de Egas Moniz
Portugal, Caparica
Egas Moniz School of Health and Science
Gonçalves, Fátima
Portugal, Lisbon
Centro Hospitalar de Lisboa Ocidental Hospital de Egas Moniz
Gottlieb., Geoffrey S.
United States, Seattle
University of Washington School of Medicine
Kupfer, Bernd
Germany, Bonn
Universität Bonn
Ruelle, Jean
Belgium, Louvain-la-neuve
Université Catholique de Louvain
Rodes, Berta
Spain, Madrid
Hospital Universitario la Paz
Soriano, Vicente Vicente
Spain, Madrid
Hospital Universitario la Paz
Wainberg, Mark A.
Canada, Montreal
Institut Lady Davis de Recherches Médicales
Taïeb, Audrey
France, Paris
Inserm
Matheron, Sophie
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
France, Paris
Université Paris Cité
Chêne, Geneviève
France, Paris
Inserm
Brun-Vézinet, Françoise
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
France, Paris
Université Paris Cité
Statistics
Citations: 31
Authors: 20
Affiliations: 15
Identifiers
Doi:
10.1128/JCM.02389-10
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Design
Cohort Study