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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus
Molecular Immunology, Volume 42, No. 1, Year 2005
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Description
The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, including one with a neutralizing titre of 0.075 nM. A phylogenetic analysis of the heavy and light chain sequences reveals that the mAbs share considerable homology. The majority of the heavy chains belong to a single Ig germline V-gene family, while considerably more sequence variation is evident in the light chain sequences. These analyses demonstrate that neutralization ability can be correlated with specific murine V H-gene alleles. For instance, one evident trend is high sequence conservation in the V H chains of the neutralizing mAbs, particularly in CDR-1 and CDR-2. The results suggest that optimization of murine mAbs for neutralization of SARS-CoV infection will likely be possible, and will aid in the development of diagnostic tools and passive treatments for SARS-CoV infection. © 2004 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Gubbins, Michael J.
Canada, Ottawa
Health Canada
Plummer, Francis Allan
Canada, Ottawa
Health Canada
Canada, Winnipeg
University of Manitoba
Dreböt, Michael A.
Canada, Ottawa
Health Canada
Canada, Winnipeg
University of Manitoba
Andonov, A.
Canada, Ottawa
Health Canada
Canada, Winnipeg
University of Manitoba
Statistics
Citations: 16
Authors: 4
Affiliations: 4
Identifiers
Doi:
10.1016/j.molimm.2004.06.032
ISSN:
01615890
Research Areas
Covid
Genetics And Genomics