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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Molecular characterization of methicillin-resistant Staphylococcus aureus isolated in Tunisia
Diagnostic Microbiology and Infectious Disease, Volume 55, No. 1, Year 2006
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Description
Community-acquired methicillin-resistant Staphylococcus aureus (MRSA) infections are an emerging problem, especially related to the production of staphylococcal toxins. In this study we investigate the phenotypic and genotypic characteristics of 72 Tunisian MRSA. Our results revealed that these strains are multiresistant. Using multiplex polymerase chain reaction, we detected staphylococcal cassette chromosome mec (SCCmec) type IV and IVA in 66 isolates. The latter are Panton-Valentine leukocidin positive. The leukotoxin genes lukE-lukD were found in most strains (92.4%). The amplification of γ-hemolysin gene was detected only in 2 MRSA isolates. Among all strains, only 1 expressed the exfoliatin A. fnbA gene was detected in 12 strains, fnbB gene in 2 strains, and both fnbA and fnbB genes in 2 other strains. The most predominant accessory gene regulator group identified was group III. Full characterization of these MRSA strains requires the association of SCCmec typing with other molecular methods such as pulsed-field gel electrophoresis, multilocus-sequence typing, and spa typing. © 2006 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Ben Nejma, Mouna
Tunisia, Monastir
Institut Supérieur de Biotechnologie de Monastir
Mastouri, Maha
Tunisia, Monastir
Laboratoire de Microbiologie
Frih, Sonia
Tunisia, Monastir
Faculté de Médecine de Monastir
Sakly, Nabil
Tunisia, Monastir
Laboratoire D'immunologie
Ben Salem, Youcef
Tunisia, Sousse
Laboratoire de Microbiologie
Nour, Mohamed
Tunisia, Monastir
Institut Supérieur de Biotechnologie de Monastir
Statistics
Citations: 36
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1016/j.diagmicrobio.2005.10.017
ISSN:
07328893
Research Areas
Genetics And Genomics
Study Locations
Tunisia