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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through modulation of oxidative stress genes expression
Chemico-Biological Interactions, Volume 192, No. 3, Year 2011
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Description
Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion and/or action. One of the most important complications of this metabolic disease is diabetic nephropathy. Hyperglycemia promotes oxidative stress and hence generation of reactive oxygen species (ROS), which is known to play a crucial role in the pathogenesis of diabetic nephropathy. Recent studies have established that metformin, an oral hypoglycemic drug, possesses antioxidant effects. However, whether metformin can protect against diabetic nephropathy has not been reported before. The overall objectives of the present study are to elucidate the potential nephroprotective effect of metformin in a rat diabetic nephropathy model and explore the exact underlying mechanism(s) involved. The effect of metformin on the biochemical changes associated with hyperglycemia induced by streptozotocin was investigated in rat kidney tissues. In addition, energy nucleotides (AMP and ATP), and Acetyl-CoA in the kidney homogenates and mitochondria, and the mRNA expression of oxidative stress and pro-inflammatory mediators were assessed. Our results showed that treatment of normoglycemic rats with metformin caused significant increase in ATP, Acetyl-CoA, and CoA-SH contents in kidney homogenates and mitochondria along with profound decrease in AMP level. On the other hand, treatment of diabetic nephropathy rats with metformin normalized all biochemical changes and the energy status in kidney tissues. At the transcriptional levels, metformin treatment caused significant restoration in diabetic nephropathy-induced oxidative stress mRNA levels, particularly GSTα, NQO1, and CAT genes, whereas inhibited TNF-α and IL-6 pro-inflammatory genes. Our data lend further credence for the contribution of metformin in the nephroprotective effect in addition to its well known hypoglycemic action. © 2011 Elsevier Ireland Ltd. All rights reserved.
Authors & Co-Authors
Alhaider, Abdulqader A.
Saudi Arabia, Riyadh
College of Pharmacy
Korashy, Hesham Mohamed
Saudi Arabia, Riyadh
College of Pharmacy
Sayed-Ahmed, Mohamed M.
Saudi Arabia, Riyadh
College of Pharmacy
Mobark, Mohammed
Saudi Arabia, Riyadh
College of Medicine
K̈foury, Hala Kassouf
Saudi Arabia, Riyadh
College of Medicine
Mansour, M. A.
Saudi Arabia, Riyadh
College of Pharmacy
Statistics
Citations: 165
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.cbi.2011.03.014
ISSN:
00092797
e-ISSN:
18727786
Research Areas
Health System And Policy
Noncommunicable Diseases