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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Characterization of a human pancreatic carcinoid in vitro: Morphology, amine and peptide storage, and secretion
Pancreas, Volume 9, No. 1, Year 1994
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Description
The study of functioning human endocrine tumors has been hampered by a lack of suitable in vitro models. We have established the first permanent cell line of a human pancreatic carcinoid tumor (BON) in culture. BON cells grow in monolayer culture and form colonies in soft agar. Injection of BON cells into nude mice produces transplantable tumors in a dose-dependent fashion. The histology of tumors in athymic mice from injection of dispersed, cultured BON cells is similar to the original histology of the resected tumor. Significant amounts of neurotensin, pancreastatin, and serotonin (5-HT) are demonstrated in the cells by radioimmunoassay (RIA) and the presence of chromogranin A, bombesin, and 5-HT is confirmed by immunocytochemistry. Numerousround and pleomorphic dense-core neurosecretory granules are present on electron microscopy. Functional receptors for acetylcholine, 5-HT, isoproterenol, and somatostatin are present on cultured cells. BON cells possess a specific transport system for uptake of 5-HT from the medium; this uptake system may be a route for regulation of autocrine effects of 5-HT on carcinoid cells. This unique human carcinoid tumor cell line should pro-vide the opportunity for new insight into the biology of carcinoid tumors and of specific intracellular mechanisms for secretagogue action in the release of amines and peptides. © 1994 Raven Press, Ltd., New York.
Authors & Co-Authors
Parekh, Dilipkumar
South Africa, Johannesburg
University of the Witwatersrand
Ishizuka, Jin
United States, Galveston
The University of Texas Medical Branch at Galveston
Townsend, Courtney M.
United States, Galveston
The University of Texas Medical Branch at Galveston
Haber, Bernard E.
United States, Galveston
University of Texas Medical Branch, Marine Biomedical Institute
Beauchamp, Robert Daniel
United States, Galveston
The University of Texas Medical Branch at Galveston
Karp, George
United States, Galveston
University of Texas Medical Branch, Marine Biomedical Institute
Kim, Sunwhe
United States, Galveston
The University of Texas Medical Branch at Galveston
Rajaraman, Srinivasan
United States, Galveston
The University of Texas Medical Branch at Galveston
Greeley, George H.
United States, Galveston
The University of Texas Medical Branch at Galveston
Thompson, James C.
United States, Galveston
The University of Texas Medical Branch at Galveston
Statistics
Citations: 123
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1097/00006676-199401000-00013
ISSN:
08853177
e-ISSN:
15364828
Research Areas
Cancer