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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Temporal similarity between candida albicans genotypes in a Tunisian neonatal intensive care unit suggests several nosocomial cross-contamination episodes
DNA and Cell Biology, Volume 31, No. 7, Year 2012
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Description
The nosocomial transmission of Candida albicans in neonatal intensive care units (NICUs) is an increasing concern and understanding the route of this transmission is critical for adequate infection control measures. The aim of our study was to assess the likeliness of nosocomial acquisition of C. albicans in the NICU of Farhat Hached hospital in Sousse (Tunisia). We genotyped 82 isolates from 40 neonates and 7 isolates from 5 health care workers (HCWs) with onychomycosis, by using CDC3 microsatellite length polymorphism (MLP) and the high-resolution melting (HRM) analysis. Combined MLP and HRM CD3 analysis led to the delineation of 12 genotypes. Five temporal clustering caused by five genotypes occurred during the study period. Three of these genotypes were isolated in both neonates and HCWs. The first clustering included 28 isolates obtained between January 2003 and May 2004 from 16 neonates and 2 HCWs. The second clustering included three isolates collected in 2004 from three neonates and two HCWs. The third clustering included 11 isolates obtained from 6 neonates and 1 HCW in 2006. The two remaining clustering could not be associated with any HCW's contamination. These results argue for the nosocomial transmission of C. albicans in our NICU. The combined MLP and HRM analysis is a rapid first approach for tracking cross-contamination. © 2012 Mary Ann Liebert, Inc.
Authors & Co-Authors
Ben Abdeljelil, Jihene
Tunisia, Sousse
Hopital Farhat Hached Sousse
Saghrouni, Fatma
Tunisia, Sousse
Hopital Farhat Hached Sousse
Cabaret, Odile
France, Maisons-alfort
Ecole Nationale Vétérinaire D'alfort
Boukadida, Jalel
Tunisia, Sousse
Hopital Farhat Hached Sousse
Bretagne, Stéphane
France, Maisons-alfort
Ecole Nationale Vétérinaire D'alfort
Ben Saïd, Moncef
Tunisia, Sousse
Hopital Farhat Hached Sousse
Statistics
Citations: 6
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1089/dna.2011.1593
ISSN:
10445498
Research Areas
Health System And Policy
Maternal And Child Health
Study Locations
Tunisia