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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
CD8
+
T cells provide an immunologic signature of tuberculosis in young children
American Journal of Respiratory and Critical Care Medicine, Volume 185, No. 2, Year 2012
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Description
The immunologic events surrounding primary Mycobacterium tuberculosis infection and development of tuberculosis remain controversial. Young children who develop tuberculosis do so quickly after first exposure, thus permitting study of immune response to primary infection and disease. We hypothesized that M. tuberculosis-specific CD81 T cells are generated in response to high bacillary loads occurring during tuberculosis. Objectives: To determine if M. tuberculosis-specific T cells are generated among healthy children exposed to M. tuberculosis and children with tuberculosis. Methods: Enzyme-linked immunosorbent spot assays were used to measure IFN-γ production in response to M.tuberculosis-specific proteins ESAT-6/CFP-10 by peripheral blood mononuclear cells and CD8 + T cells isolated from Ugandan children hospitalized with tuberculosis (n = 96) or healthy tuberculosis contacts (n = 62). Measurements and Main Results: The proportion of positive CD8 +T-cell assays and magnitude of CD8 + T-cell responses were significantly greater among young (<5 yr) tuberculosis cases compared with young contacts (P = 0.02, Fisher exact test, P = 0.01, Wilcoxon rank-sum, respectively). M. tuberculosis-specific T-cell responses measured in peripheral blood mononuclear cells were equivalent between groups. Conclusions: Among young children, M. tuberculosis-specific CD8 +T cells develop in response to high bacillary loads, as occurs during tuberculosis, andareunlikely to be found after M. tuberculosis exposure. T-cell responses measured in peripheral blood mononuclear cells are generated after M. tuberculosis exposure alone, and thus cannot distinguish exposure from disease. In young children, IFN-γ-producing M. tuberculosis-specific CD8 + T cells provide an immunologic signature of primary M. tuberculosis infection resulting in disease. Copyright © 2012 by the American Thoracic Society.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3297089/bin/supp_185_2_206__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3297089/bin/supp_185.2.206_201107-1355OC.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3297089/bin/supp_185_2_206_v2_index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3297089/bin/supp_185.2.206_Lancioni_ODS.pdf
Authors & Co-Authors
Lancioni, Christina L.
Unknown Affiliation
Nyendak, Melissa R.
Unknown Affiliation
Kiguli, Sarah
Unknown Affiliation
Zalwango, Sarah K.
Unknown Affiliation
Mori, Tomi
Unknown Affiliation
Mayanja-Kizza, Harriet
Unknown Affiliation
Balyejusa, Stephen
Unknown Affiliation
Null, Megan D.
Unknown Affiliation
Baseke, Joy
Unknown Affiliation
Mulindwa, Deo
Unknown Affiliation
Byrd, Laura
Unknown Affiliation
Swarbrick, Gwendolyn M.
Unknown Affiliation
Scott, Christine
Unknown Affiliation
Johnson, Denise F.
Unknown Affiliation
Malone, La Shaunda L.
Unknown Affiliation
Mudido-Musoke, Philipa
Unknown Affiliation
Boom, W. Henry
Unknown Affiliation
Lewinsohn, David Michael
Unknown Affiliation
Lewinsohn, Deborah Anne
Unknown Affiliation
Statistics
Citations: 69
Authors: 19
Affiliations: 7
Identifiers
Doi:
10.1164/rccm.201107-1355OC
ISSN:
1073449X
Research Areas
Maternal And Child Health