Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Challenges of using molecular serotyping for surveillance of pneumococcal disease
Journal of Clinical Microbiology, Volume 52, No. 9, Year 2014
Notification
URL copied to clipboard!
Description
Recent advances in the molecular identification and serotyping of Streptococcus pneumoniae are useful for culture-negative samples; however, there are limitations associated with these methods. We aimed to assess the value of molecular assays for invasive pneumococcal disease (IPD) surveillance in South Africa from 2010 through 2012. Nonviable isolates and culture-negative clinical specimens were tested for the lytA gene and, if positive, were serotyped, using real-time PCRs. Multinomial regression analysis was used to determine the maximum lytA cycle threshold (CT) value useful for predicting the ability to detect a serotype for the sample. The χ2 test was used to compare the prevalence of serotypes between viable/nonviable isolates and culture-negative clinical specimens. Of 11,224 IPD cases reported, 1,091 (10%) were culture-negative samples and 981 (90%) of these were lytA positive. Samples with a lytA CT value of ≥35 were significantly less likely to be serotyped. A serotype/group was determined for 87% (737/844) of samples with a lytA CT value of <35, of which 60% (443/737) were identified as individual serotypes. The serotype prevalence did not differ significantly between isolates and culture-negative specimens. Although molecular serotyping added 7% (737/11,224) serotyping data, the inability to resolve 40% of samples to single serotypes remains a challenge for sero-type-specific data analysis. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4313149/bin/supp_52_9_3271__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC4313149/bin/JCM.01061-14_zjm999093672so1.pdf
Authors & Co-Authors
Magomani, Victoria
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
School of Pathology
South Africa, Tygerberg
South African Medical Research Council
Wolter, Nicole
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
School of Pathology
South Africa, Tygerberg
South African Medical Research Council
Tempia, Stefano
South Africa, Johannesburg
National Institute for Communicable Diseases
United States, Atlanta
Centers for Disease Control and Prevention
Plessis, Mignon Du
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
School of Pathology
South Africa, Tygerberg
South African Medical Research Council
de Gouveia, Linda D.
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Tygerberg
South African Medical Research Council
von Gottberg, Anne M.
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
School of Pathology
South Africa, Tygerberg
South African Medical Research Council
Statistics
Citations: 6
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1128/JCM.01061-14
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
South Africa