Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Characterization of the fine specificity of bovine CD8 T-cell responses to defined antigens from the protozoan parasite Theileria parva
Infection and Immunity, Volume 76, No. 2, Year 2008
Notification
URL copied to clipboard!
Description
Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of developing a subunit vaccine. To facilitate further dissection of the specificity of protective CD8 T-cell responses and to assist in the assessment of responses to vaccination, we set out to identify the epitopes recognized in these T. parva antigens and their MHC restriction elements. Nine epitopes in six T. parva antigens, together with their respective MHC restriction elements, were successfully identified. Five of the cytotoxic-T-lymphocyte epitopes were found to be restricted by products of previously described alleles, and four were restricted by four novel restriction elements. Analyses of CD8 T-cell responses to five of the epitopes in groups of cattle carrying the defined restriction elements and immunized with live parasites demonstrated that, with one exception, the epitopes were consistently recognized by animals of the respective genotypes. The analysis of responses was extended to animals immunized with multiple antigens delivered in separate vaccine constructs. Specific CD8 T-cell responses were detected in 19 of 24 immunized cattle. All responder cattle mounted responses specific for antigens for which they carried an identified restriction element. By contrast, only 8 of 19 responder cattle displayed a response to antigens for which they did not carry an identified restriction element. These data demonstrate that the identified antigens are inherently dominant in animals with the corresponding MHC genotypes. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Graham, Simon P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
United Kingdom, Addlestone
Animal and Plant Health Agency
Pellé, Roger
Kenya, Nairobi
International Livestock Research Institute Nairobi
Yamage, Mat
Kenya, Nairobi
International Livestock Research Institute Nairobi
France, Paris
World Organisation for Animal Health Woah
Mwangi, Duncan M.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Honda, Yoshikazu
Kenya, Nairobi
International Livestock Research Institute Nairobi
Australia, Adelaide
Vaxine Pty Ltd
Mwakubambanya, Ramadhan
Kenya, Nairobi
International Livestock Research Institute Nairobi
Greece, Heraklion
Institute of Molecular Biology and Biotechnology of the Foundation for Research and Technology Hellas
De Villiers, Etienne P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Abuya, Evelyne
Kenya, Nairobi
International Livestock Research Institute Nairobi
Awino, Elias
Kenya, Nairobi
International Livestock Research Institute Nairobi
Gachanja, James N.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Mbwika, Ferdinand
Kenya, Nairobi
International Livestock Research Institute Nairobi
Muthiani, Anthony M.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Muriuki, Cecilia W.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Nyanjui, John K.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Onono, Fredrick O.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Germany, Hannover
Hannover Medical School
Osaso, Julius
Kenya, Nairobi
International Livestock Research Institute Nairobi
Riitho, Victor
Kenya, Nairobi
International Livestock Research Institute Nairobi
Saya, Rosemary M.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Ellis, Shirley A.
United Kingdom, Surrey
The Pirbright Institute
McKeever, Declan J.
United Kingdom, Midlothian
Moredun Research Institute
United Kingdom, Roslin
The Royal Dick School of Veterinary Studies
United Kingdom, London
Royal Veterinary College University of London
MacHugh, Niall D.
United Kingdom, Roslin
The Royal Dick School of Veterinary Studies
Gilbert, Sarah C.
United Kingdom, Oxford
The Wellcome Centre for Human Genetics
Audonnet, Jean Christophe
Germany, Ingelheim am Rhein
Boehringer Ingelheim International Gmbh
Morrison, William Ivan
United Kingdom, Roslin
The Royal Dick School of Veterinary Studies
Van Der Bruggen, Pierre
Belgium, Brussels
Ludwig Institute for Cancer Research Brussels
Taracha, Evans L.N.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Statistics
Citations: 80
Authors: 26
Affiliations: 13
Identifiers
Doi:
10.1128/IAI.01244-07
ISSN:
00199567