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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Replicative intermediates of maize streak virus found during leaf development
Journal of General Virology, Volume 91, No. 4, Year 2010
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Description
Geminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, Mastrevirus, does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR. © 2010 SGM.
Authors & Co-Authors
Erdmann, Julia B.
Germany, Stuttgart
Universität Stuttgart
Shepherd, Dionne Natalie
South Africa, Cape Town
University of Cape Town
Martin, Darren Patrick
South Africa, Cape Town
University of Cape Town
Varsani, Arvind
South Africa, Cape Town
University of Cape Town
New Zealand, Christchurch
University of Canterbury
Rybicki, Edward P.
South Africa, Cape Town
University of Cape Town
Jeske, Holger
Germany, Stuttgart
Universität Stuttgart
Statistics
Citations: 32
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1099/vir.0.017574-0
ISSN:
00221317
e-ISSN:
14652099
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study