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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
HPV detection in primary intra-oral squamous cell carcinomas - Commensal, aetiological agent or contamination?
Journal of Oral Pathology and Medicine, Volume 35, No. 2, Year 2006
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Description
BACKGROUND: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods. METHODS: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPoint™). RESULTS: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques. CONCLUSIONS: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method. © Blackwell Munksgaard 2006. All rights reserved.
Authors & Co-Authors
Boy, Sonja C.
South Africa, Pretoria
University of Pretoria
Rensburg, Estrelita Janse Van
South Africa, Pretoria
University of Pretoria
Engelbrecht, Susan
South Africa, Stellenbosch
Stellenbosch University
Dreyer, Leonora
South Africa, Pretoria
University of Pretoria
van Heerden, Marlene B.
South Africa, Pretoria
University of Pretoria
van Heerden, Willie F.P.
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 106
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1111/j.1600-0714.2006.00385.x
ISSN:
09042512
e-ISSN:
16000714
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study