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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Molecular characterization of Equine Infectious Anemia Viruses using targeted sequence enrichment and next generation sequencing
Virology, Volume 537, Year 2019
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Description
Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system with Illumina Next Generation Sequencing to characterize the proviral DNA of Equine Infectious Anemia Virus (EIAV) from asymptomatic horses. This approach allows a direct sequencing of the EIAV whole genome without cloning or amplification steps and we could obtain for the first time the complete genomic DNA sequences of French EIAV strains. We analyzed their phylogenetic relationship and genetic variability by comparison with 17 whole EIAV genome sequences from different parts of the world. The results obtained provide new insights into the molecular detection of EIAV and genetic diversity of European viral strains. © 2019
Authors & Co-Authors
Berthet, Nicolas
France, Paris
Institut Pasteur, Paris
France, Paris
Génétique Moléculaire Des Virus à Arn
Lecouturier, Fanny
France, Goustranville
Anses-laboratory for Animal Health in Normandy
Gaudaire, Delphine
France, Goustranville
Anses-laboratory for Animal Health in Normandy
Hans, Aymeric
France, Goustranville
Anses-laboratory for Animal Health in Normandy
Statistics
Citations: 9
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1016/j.virol.2019.08.016
ISSN:
00426822
Research Areas
Cancer
Genetics And Genomics