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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Development and validation of a PCR-RFLP test to identify African Rhipicephalus (Boophilus) ticks
Acta Tropica, Volume 114, No. 1, Year 2010
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Description
The cattle tick Rhipicephalus (Boophilus) microplus has recently invaded West Africa and caused anxiety amongst farmers in Ivory Coast, as livestock production was severely affected. The introduction of this tick species has remained unnoticed for several years, as all the members of this genus are very similar in appearance. To overcome the cumbersome morphological identification of the four closely related R. (Boophilus) spp. in the region, a PCR-RFLP test, based on a part of the second internal transcribed spacer ribosomal DNA (ITS2), was developed. The molecular tool was successfully validated with a large number of ticks recently collected from West Africa and that were identified both morphologically and genetically. The tool developed is simple, fast, reliable and reproducible; hence it can be routinely applied for species identification. © 2010 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Lempereur, Laetitia
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Geysen, Dirk M.
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Madder, Maxime
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Statistics
Citations: 41
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/j.actatropica.2010.01.004
ISSN:
0001706X
Research Areas
Genetics And Genomics
Mental Health
Study Locations
Multi-countries
Ivory Coast