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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Poor immunogenicity of BCG in helminth infected population is associated with increased in vitro TGF-β production
Vaccine, Volume 26, No. 31, Year 2008
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Description
The only vaccine available against tuberculosis (TB), BCG, so effective in experimental animal models, has been under scrutiny for a long time owing to its variable efficacy against pulmonary tuberculosis in adults. In this study, we evaluated whether anti-helminthic therapy prior to BCG vaccination could increase the immunogenicity of BCG vaccination in helminth infected population. We recruited volunteers with evidence of prior mycobacterial infection and who were asymptomatic carriers of helminths. The subjects were randomized to receive either anti-helminthic drugs or placebo. Three months later, BCG vaccination was administered to volunteers. Mycobacterial antigen-specific cytokine responses were assessed 2 months after vaccination. The results show that peripheral blood mononuclear cells obtained from the placebo group were found to have a lower frequency of IFN-γ (129 vs 191, p = 0.03) and IL-12 (149 vs 243, p = 0.013) producing cells per 2 × 105 PBMC (peripheral blood mononuclear cells) when stimulated in vitro with a mycobacterial antigen mixture (purified protein derivative (PPD)) compared to those from the dewormed group. On the other hand the placebo group had higher frequency of TGF-β producing cells in response to PPD (152 vs 81.3, p = 0.002) or the T cell mitogen concanavalin A (Con A) (210 vs 157, p = 0.03). However, no detectable IL-4 or IL-5 producing cells were observed when cells were stimulated with PPD. Comparable numbers of both cytokine producing cells were induced in both groups upon stimulation with concanavalin A (IL-4 217 vs 191, p = 0.08) and IL-5 (131 vs 103, p = 0.14). The data presented here demonstrate that chronic worm infection reduces the immunogenicity of BCG in humans and this was associated with increased TGF-β production but not with enhanced Th2 immune response. © 2008 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Elias, Daniel
Ethiopia, Addis Ababa
Armauer Hansen Research Institute
Sweden, Stockholm
Karolinska Institutet
Britton, Sven F.F.
Sweden, Stockholm
Karolinska Institutet
Aseffa, Abraham
Ethiopia, Addis Ababa
Armauer Hansen Research Institute
Engers, Howard D.
Ethiopia, Addis Ababa
Armauer Hansen Research Institute
Akuffo, Hannah Opokua
Sweden, Stockholm
Karolinska Institutet
Statistics
Citations: 204
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/j.vaccine.2008.04.083
ISSN:
0264410X
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study