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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A molecular approach for identification of paramphistomes from Africa and Asia
Veterinary Parasitology, Volume 174, No. 3-4, Year 2010
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Description
Paramphistomiasis is a disease of domestic and wild ruminants caused by some digenetic trematodes of the superfamily Paramphistomoidea. Human infections with certain species of paramphistomes have been reported. Given their ubiquity and their abundance within hosts, it seems likely that the importance of these worms is underestimated globally. Based on the reported difficulties of morphological identification of paramphistomes, the development of alternative approaches to better delineate species is needed. Paramphistome samples examined during this study were cercariae, rediae from snail hosts and adult flukes obtained from slaughterhouses from Kenya, Tanzania, Egypt and Nepal. The results confirm that ITS2 is a good molecular marker for paramphistome identification and can be used to determine the affiliation of samples among the different taxa of Paramphistomoidea. Our 22 samples include eight species of paramphistomes. Ten samples of paramphistome larvae (cercariae or rediae) yielded seven species and the 12 samples of adult flukes from bovines yielded four, only one of which was not also represented among the larvae. We found three cases in which the sequences for adult worms and larvae matched, thus revealing the hosts involved locally in those particular life cycles. We interpret our specimens to include five species from Kenya, two from Nepal, two from Tanzania and one from Egypt. Bulinus forskalii was found infected with three species of paramphistomes. The results of the present study will help construct a framework to facilitate identification and eventually control of this underestimated cosmopolitan group of digeneans. © 2010 Elsevier B.V.
Authors & Co-Authors
Lotfy, Wael Mohamed
Egypt, Alexandria
Alexandria University
Brant, Sara Vanessa
United States, Albuquerque
The University of new Mexico
Ashmawy, Karam Imam
Egypt, Alexandria
Faculty of Veterinary Medicine
Devkota, Ramesh
United States, Albuquerque
The University of new Mexico
Nepal, Kathmandu
Centre for Biological Conservation
Mkoji, Gerald M.
Kenya, Nairobi
Kenya Medical Research Institute
Loker, Eric Samuel
United States, Albuquerque
The University of new Mexico
Statistics
Citations: 60
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1016/j.vetpar.2010.08.027
ISSN:
03044017
Study Locations
Egypt
Kenya
Tanzania