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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains
Journal of Antimicrobial Chemotherapy, Volume 64, No. 2, Year 2009
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Description
Objectives: Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone. Methods: A totalof 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene. Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris. Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15. © The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Authors & Co-Authors
Clermont, Olivier
France, Paris
Inserm
Dhanji, Hiran
United Kingdom, London
Public Health England
Upton, Mathew
United Kingdom, Manchester
The University of Manchester
Gibreel, Tarek M.
United Kingdom, Manchester
The University of Manchester
Fox, Andrew J.
United Kingdom, London
Public Health England
Boyd, David A.
Canada, Winnipeg
National Microbiology Laboratory
Mulvey, Michael Richard
Canada, Winnipeg
National Microbiology Laboratory
Nordmann, Patrice L.
France, Gif-sur-yvette
Université Paris-saclay
Ruppé, Etienne
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Sarthou, Jean Louis
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Frank, Thierry
France, Paris
Sorbonne Université
Central African Republic, Bangui
Institut Pasteur de Bangui
Vimont, Sophie
France, Paris
Sorbonne Université
France, Paris
Hôpital Tenon
Arlet, Guillaume A.
France, Paris
Sorbonne Université
France, Paris
Hôpital Tenon
Branger, Catherine
France, Paris
Inserm
France, Colombes
Hopital Louis-mourier
Woodford, Neil
United Kingdom, London
Public Health England
Denamur, Erick
France, Paris
Inserm
Statistics
Citations: 376
Authors: 16
Affiliations: 10
Identifiers
Doi:
10.1093/jac/dkp194
ISSN:
03057453
e-ISSN:
14602091
Research Areas
Cancer
Genetics And Genomics