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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Rapid investigation of hepatitis A virus outbreak by single strand conformation polymorphism analysis
Journal of Medical Virology, Volume 76, No. 2, Year 2005
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Description
Investigation of hepatitis A virus (HAV) outbreaks often implies nucleotide sequence analysis. As an alternative method for the identification of related strains, single strand conformation polymorphism method (SSCP) was compared to sequence analysis. Twenty-three strains from sporadic and outbreak cases were studied retrospectively. SSCP, sequence identity and phylogenetic analyses were conducted on a 267 bp fragment of the VP1-2A variable region. The results of SSCP pattern comparison and sequence identity were highly correlated (r = 0.92, P < 0.001). If SSCP showed similar patterns, the VP1-2A fragments had a high and significant probability to have a sequence identity over 99.6%. Results were concordant for outbreak strains. The only discordant result concerned a cluster of three sporadic cases evidenced by phylogenetic analysis while SSCP showed similar patterns for only two of these three cases. A prospective SSCP analysis of a recent HAV outbreak confirmed the reliability of this technique. SSCP may thus provide a rapid and cost-effective tool for preliminary investigation of HAV outbreaks, before undertaking exhaustive nucleotide sequence analysis. © 2005 Wiley-Liss, Inc.
Authors & Co-Authors
Mackiewicz, Vincent
France, Villejuif
Hopital Paul-brousse
Roque-Afonso, Anne Marie
France, Villejuif
Hopital Paul-brousse
Marchadier, Eric
France, Villejuif
Hopital Paul-brousse
Nicand, Élisabeth
France, Paris
Laboratoire de Biologie Clinique
Fki-Berrajah, Lamia
Tunisia, Sfax
Chu Habib Bourguiba
Dussaix, Élisabeth
France, Villejuif
Hopital Paul-brousse
Statistics
Citations: 10
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1002/jmv.20355
ISSN:
01466615
Research Areas
Infectious Diseases
Study Design
Cohort Study