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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana
Molecular Systems Biology, Volume 6, Article 397, Year 2010
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Description
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)ĝ€"cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A- and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA;1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDKĝ€"cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants. © 2010 EMBO and Macmillan Publishers Limited.
Authors & Co-Authors
Eeckhout, Dominique
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
de Bodt, Stefanie
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Maere, Steven
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Laukens, Kris
Belgium, Antwerpen
Universiteit Antwerpen
Ferreira, Paulo Cavalcanti Gomes
Brazil, Rio de Janeiro
Universidade Federal do Rio de Janeiro
Meyer, Christian
France, Versailles
Centre de Recherche Île-de-france - Versailles-grignon
Van de Peer, Y.
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Hilson, Pierre
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
de Veylder, Lieven D.
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Inze ́, Dirk G.
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Witters, Erwin
Belgium, Antwerpen
Universiteit Antwerpen
Belgium, Mol
Vlaamse Instelling Voor Technologisch Onderzoek
de Jaeger, Geert D.
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Statistics
Citations: 287
Authors: 12
Affiliations: 9
Identifiers
Doi:
10.1038/msb.2010.53
ISSN:
17444292
Study Approach
Qualitative