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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evaluation of a rapid dot-elisa as a field test for the diagnosis of cystic hydatid disease
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 85, No. 6, Year 1991
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Description
A rapid dot enzyme-linked immunosorbent assay (dot-ellsa) was developed as a field test for the diagnosis of cystic hydatid disease caused by Echinococcus granulosus. The 30 min test was based on the detection of antibodies to antigen B of hydatid fluid and was carried put using 50 μl of whole blood in a field assessment in the Turkana region of north-west Kenya. Initial laboratory studies showed antigen B to be preferable to crude cyst fluid, with 94% sensitivity and 90·3% specificity for Echinococcus infections. The field test was rapid, inexpensive and simple to perform and is considered to be a useful back-up to ultrasound scanning. © 1991.
Authors & Co-Authors
Rogan, Michael Thomas
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Craig, Phillip Simon
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Zeyhle, Eberhard E.
Kenya, Nairobi
Amref Health Africa
Römig, Thomas J.
Kenya, Nairobi
Amref Health Africa
Lubano, G. M.
Kenya, Nairobi
Kenya Medical Research Institute
Deshan, Liu
China, Lanzhou
Lanzhou Medical College
Statistics
Citations: 87
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/0035-9203(91)90451-4
ISSN:
00359203
Study Locations
Kenya