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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Genetic Characterization of Cryptosporidium spp. in Diarrhoeic Children from Four Provinces in South Africa
Zoonoses and Public Health, Volume 60, No. 2, Year 2013
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Description
The diversity of Cryptosporidium at species, subtype family and subtype level in diarrhoeic children was investigated in four provinces in South Africa. A total of 442 stool samples from children <5years of age were collected under a large rotavirus surveillance programme and analysed by Ziehl-Neelsen acid-fast staining. Fifty-four (12.2%) were positive for Cryptosporidium, of which 25 were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and DNA sequence analyses of the 18S rRNA gene. The majority of genotyped specimens were identified as C. hominis (76%), and a high genetic diversity was found with five different C. hominis subtype families (Ia, Ib, Id, Ie and If). Cryptosporidium parvum was found in 20% of the isolates, and three subtype families were identified (IIc, IIe and IIb), with subtype family IIc being the most common. One specimen was identified as C. meleagridis of the subtype family IIId. These results are in accordance with findings from other developing countries and report for the first time the presence in South Africa of C. meleagridis, various subtypes of C. parvum and the subtype family Ie of C. hominis. The results suggest that C. hominis and anthroponotic C. parvum subtypes are the major cause of cryptosporidiosis in South Africa. Further molecular studies are needed to better understand the epidemiology and public health importance of Cryptosporidium in humans in South Africa. © 2012 Blackwell Verlag GmbH.
Authors & Co-Authors
Abu Samra, Nada
South Africa, Pretoria
University of Pretoria
Thompson, Peter N.
South Africa, Pretoria
University of Pretoria
Jori, Ferrán
South Africa, Pretoria
University of Pretoria
Frean, John Andrew
South Africa, Johannesburg
University of the Witwatersrand
Poonsamy, Bhavani
South Africa, Johannesburg
University of the Witwatersrand
Du Plessis, Desiree C.
South Africa, Johannesburg
University of the Witwatersrand
Mogoye, Benjamin
South Africa, Johannesburg
University of the Witwatersrand
Xiao, Lihua
United States, Atlanta
National Center for Emerging and Zoonotic Infectious Diseases
Statistics
Citations: 38
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1111/j.1863-2378.2012.01507.x
ISSN:
18631959
e-ISSN:
18632378
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health
Study Locations
South Africa