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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Nonhuman primate retroviruses from Cambodia: High simian foamy virus prevalence, identification of divergent STLV-1 strains and no evidence of SIV infection
Infection, Genetics and Evolution, Volume 18, Year 2013
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Description
Nonhuman primates (NHPs) carry retroviruses such as simian immunodeficiency viruses (SIV), simian T-cell lymphotropic viruses (STLV) and simian foamy viruses (SFV). Here, we revisited NHPs from Cambodia to assess the prevalence and diversity of these retroviruses using updated viral detection tools. We screened blood from 118 NHPs consisting of six species (Macaca fascicularis (n= 91), Macaca leonine (n= 8), Presbytis cristata (n= 3), Nycticebus coucang (n= 1), Hylobates pileatus (n= 14), and Pongo pygmaeus) (n= 1) by using a Luminex-based multiplex serology assay that allows the detection of all known SIV/HIV and SFV lineages. We also used highly sensitive PCR assays to detect each simian retrovirus group. Positive PCR products were sequenced and phylogenetically analyzed to infer evolutionary histories.Fifty-three of 118 (44.9%) NHPs tested positive for SFV by serology and 8/52 (15.4%), all from M. fascicularis, were PCR-confirmed. The 8 novel SFV sequences formed a highly supported distinct lineage within a clade composed of other macaque SFV. We observed no serological or molecular evidence of SIV infection among the 118 NHP samples tested. Four of 118 (3.3%) NHPs were PCR-positive for STLV, including one M. fascicularis, one P. cristata, and two H. pileatus. Phylogenetic analyses revealed that the four novel STLV belonged to the PTLV-1 lineage, outside the African radiation of PTLV-1, like all Asian PTLV identified so far. Sequence analysis of the whole STLV-1 genome from a H. pileatus (C578_. Hp) revealed a genetic structure characteristic of PTLV. Similarity analysis comparing the STLV-1 (C578_. Hp) sequence with prototype PTLVs showed that C578_. Hp is closer to PTLV-1s than to all other types across the entire genome. In conclusion, we showed a high frequency of SFV infection but found no evidence of SIV infection in NHPs from Cambodia. We identified for the first time STLV-1 in a P. cristata and in two H. pileatus. © 2013 Elsevier B.V.
Authors & Co-Authors
Ayouba, Ahidjo
France, Montpellier
Université de Montpellier
Duval, Linda
France, Paris
Museum National D'histoire Naturelle
Liégeois, Florian
France, Montpellier
Université de Montpellier
Ngin, Sopheak
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Ahuka-Mundeke, Steve
France, Montpellier
Université de Montpellier
Switzer, William M.
United States, Atlanta
Centers for Disease Control and Prevention
Delaporte, Éric
France, Montpellier
Université de Montpellier
Ariey, Frédéric
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Peeters, Martine F.
France, Montpellier
Université de Montpellier
Nerrienet, Eric
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Statistics
Citations: 19
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.meegid.2013.04.015
ISSN:
15677257
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study