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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Comprehensive Characterization of Protein Turnover by Comparative SILAC Labeling Analysis in 3T3-L1
Journal of Proteome Research, Volume 22, No. 6, Year 2023
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Description
A balance between the synthesis and degradation of proteins is referred to as protein turnover, which is crucial for cellular protein homeostasis. Proteome-wide analysis of protein turnover in adipocytes, which are well-known for their role in energy storage and their link to obesity and metabolism disorders, is yet to be conducted. Thus, with this objective in mind, our investigation utilized a comparative analysis of time-dependent SILAC labeling to assess protein turnover in 3T3-L1 adipocytes, spanning a period of 0 to 144 h. We observed that relatively faster or slower protein half-lives in several protein groups were associated with the PPARγ signaling pathway, energy metabolism, extracellular matrix, ubiquitin-proteasome system, RNA splicing, Golgi complex, and lysosome. It is anticipated that these protein half-life profiles will provide greater clarity on the life cycle of adipocyte proteome and shed light on how they maintain protein homeostasis. © 2023 American Chemical Society
Authors & Co-Authors
Romero, Atilio Reyes
Qatar, Doha
Qatar Foundation
Chouchane, Lotfi
Qatar, Doha
Qatar Foundation
Schmidt, Frank
Qatar, Doha
Qatar Foundation
Statistics
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1021/acs.jproteome.2c00763
ISSN:
15353893
Research Areas
Noncommunicable Diseases