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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Barley has two peroxisomal ABC transporters with multiple functions in β-oxidation
Journal of Experimental Botany, Volume 65, No. 17, Year 2014
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Description
In oilseed plants, peroxisomal β-oxidation functions not only in lipid catabolism but also in jasmonate biosynthesis and metabolism of pro-auxins. Subfamily D ATP-binding cassette (ABC) transporters mediate import of β-oxidation substrates into the peroxisome, and the Arabidopsis ABCD protein, COMATOSE (CTS), is essential for this function. Here, the roles of peroxisomal ABCD transporters were investigated in barley, where the main storage compound is starch. Barley has two CTS homologues, designated HvABCD1 and HvABCD2, which are widely expressed and present in embryo and aleurone tissues during germination. Suppression of both genes in barley RNA interference (RNAi) lines indicated roles in metabolism of 2,4-dichlorophenoxybutyrate (2,4-DB) and indole butyric acid (IBA), jasmonate biosynthesis, and determination of grain size. Transformation of the Arabidopsis cts-1 null mutant with HvABCD1 and HvABCD2 confirmed these findings. HvABCD2 partially or completely complemented all tested phenotypes of cts-1. In contrast, HvABCD1 failed to complement the germination and establishment phenotypes of cts-1 but increased the sensitivity of hypocotyls to 100 μM IBA and partially complemented the seed size phenotype. HvABCD1 also partially complemented the yeast pxa1/pxa2δ mutant for fatty acid β-oxidation. It is concluded that the core biochemical functions of peroxisomal ABC transporters are largely conserved between oilseeds and cereals but that their physiological roles and importance may differ. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Authors & Co-Authors
Mendiondo, Guillermina Mónica
United Kingdom, Nottingham
University of Nottingham
Medhurst, Anne
United Kingdom, Nottingham
University of Nottingham
van Roermund, Carlo W.T.
Netherlands, Amsterdam
Universiteit Van Amsterdam
Zhang, Xuebin
United Kingdom, Harpenden
Rothamsted Research
Australia, Melbourne
Agribio, Centre for Agribioscience
Devonshire, Jean
United Kingdom, Harpenden
Rothamsted Research
Scholefield, Duncan
United Kingdom, Nottingham
University of Nottingham
Fernández, José
United Kingdom, Nottingham
University of Nottingham
Axcell, Barry C.
South Africa, Sandton
Sabmiller Plc
Ramsay, Luke D.
United Kingdom, Aberdeen
The James Hutton Institute
Waterham, Hans R.
Netherlands, Amsterdam
Universiteit Van Amsterdam
Waugh, Robbie
United Kingdom, Aberdeen
The James Hutton Institute
Theodoulou, Frederica L.
United Kingdom, Harpenden
Rothamsted Research
Holdsworth, Michael John
United Kingdom, Nottingham
University of Nottingham
United States, Upton
Brookhaven National Laboratory
Statistics
Citations: 24
Authors: 13
Affiliations: 7
Identifiers
Doi:
10.1093/jxb/eru243
ISSN:
00220957
e-ISSN:
14602431